Rabbit Cdc40 Polyclonal Antibody | anti-Cdc40 antibody

Cdc40 (S209) Polyclonal Antibody

Cat. Num. AAA3003686

• Gene Names: CDC40; EHB3; PRP17; PRPF17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cdc40; Polyclonal Antibody; Cdc40 (S209) Polyclonal Antibody; Pre-mRNA-processing factor 17; Cell division cycle 40 homolog; EH-binding protein 3; Ehb3; PRP17 homolog; hPRP17; CDC40; EHB3; PRP17; PRPF17; anti-Cdc40 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cdc40 (S209) polyclonal antibody detects endogenous levels of Cdc40 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 579

• Applicable Applications for anti-Cdc40 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human Cdc40.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cdc40 (S209) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:CT26 whole cell lysate (10ug) Lane4:The Brain tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cdc40 (S209) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-Cdc40 antibody

Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40.

NCBI and Uniprot Product Information

• NCBI GI #: 7706657
• NCBI GeneID: 51362
• NCBI Accession #: NP_056975.1
• NCBI GenBank Nucleotide #: NP_056975.1
• UniProt Accession #: O60508
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: pre-mRNA-processing factor 17
• NCBI Official Synonym Full Names: cell division cycle 40
• NCBI Official Symbol: CDC40
• NCBI Official Synonym Symbols: EHB3; PRP17; PRPF17
• NCBI Protein Information: pre-mRNA-processing factor 17
• UniProt Protein Name: Pre-mRNA-processing factor 17
• UniProt Gene Name: CDC40
• UniProt Synonym Gene Names: EHB3; PRP17; PRPF17; Ehb3; hPRP17
• UniProt Entry Name: PRP17_HUMAN

NCBI Description

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC40: Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing.

Protein type: RNA splicing; Spliceosome; Motility/polarity/chemotaxis; RNA processing

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; spliceosome

Molecular Function: protein binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription