Rabbit HSP90B1 Polyclonal Antibody | anti-HSP90B1 antibody

HSP90B1, NT (HSP90B1, GRP94, TRA1, Endoplasmin, 94kD glucose-regulated protein, Heat shock protein 90kD beta member 1, Tumor rejection antigen 1, gp96 homolog) (FITC)

Cat. Num. AAA6308620

• Gene Names: HSP90B1; ECGP; GP96; TRA1; GRP94
• Reactivity: Human, Hamster, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HSP90B1; Polyclonal Antibody; NT (HSP90B1; GRP94; TRA1; Endoplasmin; 94kD glucose-regulated protein; Heat shock protein 90kD beta member 1; Tumor rejection antigen 1; gp96 homolog) (FITC); gp96 homolog; anti-HSP90B1 antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSP90B1. Species Crossreactivity: hamster, mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HSP90B1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 16-43 in the N-terminal region of human HSP90B1. Species sequence homology: bovine, chicken, monkey, porcine, rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2, CHO, K562, HeLa cell line and mouse liver tissue lysates (35ug/lane) usingMBS6006433. This demonstrates that MBS6006433 detected the HSP90B1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung using MBS6006433 carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006433 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS6006433 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with NCI-H460 cells using MBS6006433 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-HSP90B1 antibody

Antibodies; Heat Shock Proteins

References

Koo, B.H., et al. J. Biol. Chem. 285(1):197-205(2010). Suriano, R., et al. Glycobiology 19(12):1427-1435(2009). Lev, A., et al. J. Immunol. 183(7):4205-4210(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507677
• NCBI GeneID: 7184
• NCBI Accession #: NP_003290.1
• NCBI GenBank Nucleotide #: NM_003299.2
• UniProt Accession #: P14625; Q96A97
• Molecular Weight: 92,469 Da
• NCBI Official Full Name: endoplasmin
• NCBI Official Synonym Full Names: heat shock protein 90kDa beta (Grp94), member 1
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: ECGP; GP96; TRA1; GRP94
• NCBI Protein Information: endoplasmin; tumor rejection antigen 1; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; endothelial cell (HBMEC) glycoprotein; heat shock protein 90 kDa beta member 1; stress-inducible tumor rejection antigen gp96
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_HUMAN

NCBI Description

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Secreted; Apoptosis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q24.2-q24.3

Cellular Component: endoplasmic reticulum membrane; focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region; cytosol; extracellular matrix; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; midbody; nucleus

Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; unfolded protein response; regulation of phosphoprotein phosphatase activity; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; sequestering of calcium ion; toll-like receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of apoptosis