Typical Testing Data/Standard Curve (for reference only)
Mouse Epididymal secretory glutathione peroxidase (GPX5) ELISA Kit | GPX5 elisa kit
Mouse Epididymal secretory glutathione peroxidase (GPX5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GPX5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GPX5 antibody and an GPX5-HRP conjugate. The assay sample and buffer are incubated together with GPX5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPX5 concentration since GPX5 from samples and GPX5-HRP conjugate compete for the anti-GPX5 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPX5 from the sample, fewer sites are left to bind GPX5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPX5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the glutathione peroxidase family. It is specifically expressed in the epididymis in the mammalian male reproductive tract, and is androgen-regulated. Unlike mRNAs for other characterized glutathione peroxidases, this mRNA does not contain a selenocysteine (UGA) codon. Thus, the encoded protein is selenium-independent, and has been proposed to play a role in protecting the membranes of spermatozoa from the damaging effects of lipid peroxidation and/or preventing premature acrosome reaction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
GPX5: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - arachidonic acid; Secreted; Secreted, signal peptide; Oxidoreductase; Other Amino Acids Metabolism - glutathione; EC 1.11.1.9
Chromosomal Location of Human Ortholog: 6p22.1
Cellular Component: extracellular space
Molecular Function: glutathione peroxidase activity
Biological Process: lipid metabolic process; response to oxidative stress
Research Articles on GPX5
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Product Notes
The Mouse GPX5 gpx5 (Catalog #AAA7230990) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230990 ELISA Kit recognizes Mouse GPX5. It is sometimes possible for the material contained within the vial of "Epididymal secretory glutathione peroxidase (GPX5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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