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IHC (Immunohistochemistry)
(MLF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human, mouse MLF1 Polyclonal Antibody | anti-MLF1 antibody
MLF1 Antibody (C-term)
Reactivity
Human, mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MLF1, Antibody; MLF1 Antibody (C-term); Myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1; MLF1; anti-MLF1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human MLF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
215-244
Sequence Length
243
Applicable Applications for anti-MLF1 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(MLF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(MLF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of MLF1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MLF1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(MLF1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MLF1 antibody detected the MLF1 protein (arrow).)
WB (Western Blot)
(MLF1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MLF1 antibody detected the MLF1 protein (arrow).)
WB (Western Blot)
(Western blot analysis of MLF1 (arrow) using rabbit polyclonal MLF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.)
WB (Western Blot)
(Western blot analysis of MLF1 (arrow) using rabbit polyclonal MLF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.)
Related Product Information for anti-MLF1 antibody
MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Product Categories/Family for anti-MLF1 antibody
References
Li,Z.F., J. Neurol. Sci. 264 (1-2), 77-86 (2008)
Yoneda-Kato,N., EMBO J. 24 (9), 1739-1749 (2005)
Yoneda-Kato,N., EMBO J. 24 (9), 1739-1749 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30627
NCBI Official Full Name
myeloid leukemia factor 1 isoform 2
NCBI Official Synonym Full Names
myeloid leukemia factor 1
NCBI Official Symbol
MLF1
NCBI Protein Information
myeloid leukemia factor 1
UniProt Protein Name
Myeloid leukemia factor 1
UniProt Gene Name
MLF1
UniProt Entry Name
MLF1_HUMAN
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Product Notes
The MLF1 mlf1 (Catalog #AAA288025) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 215-244. The MLF1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MLF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MLF1 mlf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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