Rabbit anti-Human CD86 Polyclonal Antibody | anti-CD86 antibody

CD86 Antibody

Cat. Num. AAA154468

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: CD86; Polyclonal Antibody; CD86 Antibody; CD86 molecule; B70; B7-2; B7.2; LAB72; CD28LG2; anti-CD86 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least five isoforms of CD86 are known to exist; this antibody will detect all five isoforms.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 329

• Applicable Applications for anti-CD86 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86.; The immunogen is located within amino acids 160 - 210 of CD86.
• Buffer: CD86 Antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles.; Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CD86 in (A) Daudi and (B) Raji cell lysate with CD86 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CD86 in human lymph node tissue with CD86 antibody at 2 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of CD86 in human lymph node tissue with CD86 antibody at 20 μg/ml.Red: CD86 Antibody (8681)Blue: DAPI staining)

Related Product Information for anti-CD86 antibody

CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Product Categories/Family for anti-CD86 antibody

Cell Cycle; Cancer; Immunology; Immune Checkpoint

References

1. Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400. 2. Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

NCBI and Uniprot Product Information

• NCBI GI #: 332634934
• NCBI GeneID: 942
• NCBI Accession #: NP_787058
• NCBI GenBank Nucleotide #: NM_175862.4
• UniProt Accession #: P42082; Q13655; Q6FHB1; Q6GTS4; Q7M4L5; A0N0P0; B7Z2F3; B7Z702; E7ETN5; E9PC27; P42081
• Molecular Weight: Predicted: 36 kDa; Observed: 68 kDa
• NCBI Official Full Name: T-lymphocyte activation antigen CD86 isoform 1
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; receptor activity

Biological Process: cell-cell signaling; immune response; phosphoinositide-mediated signaling; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; T cell activation; T cell costimulation