Human Homeobox protein OTX2 ELISA Kit | OTX2 elisa kit

Human Homeobox protein OTX2 ELISA Kit

Cat. Num. AAA761722

• Gene Names: OTX2; CPHD6; MCOPS5
• Reactivity: Human
• Synonyms: Homeobox protein OTX2; Human Homeobox protein OTX2 ELISA Kit; OTX2/homeobox protein OTX2/MCOPS5/orthodenticle homeobox 2/Orthodenticle homolog 2/orthodenticle homolog 2 (Drosophila); OTX2 elisa kit

• Reactivity: Human
• Sequence Length: 297

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for OTX2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human OTX2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OTX2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OTX2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human OTX2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 395455075
• NCBI GeneID: 5015
• NCBI Accession #: NP_001257454.1
• NCBI GenBank Nucleotide #: NM_001270525.1
• Molecular Weight: 32,406 Da
• NCBI Official Full Name: homeobox protein OTX2 isoform a
• NCBI Official Synonym Full Names: orthodenticle homeobox 2
• NCBI Official Symbol: OTX2
• NCBI Official Synonym Symbols: CPHD6; MCOPS5
• NCBI Protein Information: homeobox protein OTX2
• UniProt Protein Name: Homeobox protein OTX2
• Protein Family: Homeobox protein
• UniProt Gene Name: OTX2
• UniProt Entry Name: OTX2_HUMAN

NCBI Description

This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]

Uniprot Description

OTX2: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. Expressed in brain. Belongs to the paired homeobox family. Bicoid subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; growth cone; nucleus; protein complex

Molecular Function: eukaryotic initiation factor 4E binding; protein binding; protein heterodimerization activity

Biological Process: axon guidance; cell fate specification; diencephalon morphogenesis; dorsal/ventral pattern formation; endoderm development; eye photoreceptor cell fate commitment; forebrain development; inner ear morphogenesis; metencephalon development; midbrain development; neuron fate determination; positive regulation of embryonic development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of fibroblast growth factor receptor signaling pathway; regulation of smoothened signaling pathway; somite rostral/caudal axis specification; transcription from RNA polymerase II promoter

Disease: Microphthalmia, Syndromic 5; Pituitary Hormone Deficiency, Combined, 6