CD86/B7-2 recombinant protein

Human CD86/B7-2 protein, His tag (Animal-Free)

Cat. Num. AAA9719056

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD86/B7-2; Human CD86/B7-2 protein; His tag (Animal-Free); T-lymphocyte activation antigen CD86; Activation B7-2 antigen; CD86; CD86/B7-2 recombinant protein

• Host: HEK293 cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4.
• Sequence Positions: 230aa
• Sequence: Met1-Pro247

• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from human CD86 (NP_787058) (Met1-Pro247) was expressed with a 6 His tag at the C-terminus.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD86/B7-2 protein using the buffer we provided not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Human CD86 protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig. SDS-PAGE analysis of Human CD86/B7-2 protein, His tag.)

Related Product Information for CD86/B7-2 recombinant protein

Recombinant Human CD86/B7-2 protein expressed in HEK293 Cells.
Background: Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Product Categories/Family for CD86/B7-2 recombinant protein

Cell Culture

NCBI and Uniprot Product Information

• NCBI GI #: 332634934
• NCBI GeneID: 942
• NCBI Accession #: NP_787058
• NCBI GenBank Nucleotide #: NM_175862.4
• UniProt Accession #: P42081; Q13655; Q6FHB1; Q6GTS4; Q7M4L5; A0N0P0; B7Z2F3; B7Z702; E7ETN5; E9PC27
• Molecular Weight: Predicted: 36 kDa; Observed: 68 kDa
• NCBI Official Full Name: T-lymphocyte activation antigen CD86 isoform 1
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2
• UniProt Entry Name: CD86_HUMAN

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; receptor binding

Biological Process: positive regulation of lymphotoxin A biosynthetic process; negative regulation of T cell anergy; T cell activation; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; response to yeast; defense response to virus; aging; response to drug; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response