Human P-Selectin (SELP) CLIA Kit | SELP clia kit

Human P-Selectin (SELP) CLIA Kit

Cat. Num. AAA2708808

• Gene Names: SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: P-Selectin (SELP); Human P-Selectin (SELP) CLIA Kit; P-Selectin; CD62P; Selectin; Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane; SELP clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of P-Selectin (SELP).; No significant cross-reactivity or interference between P-Selectin (SELP) and analogues was observed.
• Sequence Length: 830

• Applicable Applications for SELP clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 4.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P-Selectin (SELP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P-Selectin (SELP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to P-Selectin (SELP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to P-Selectin (SELP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the P-Selectin (SELP) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 183389
• NCBI GeneID: 6403
• UniProt Accession #: P16109
• NCBI Official Full Name: granule membrane protein-140
• NCBI Official Synonym Full Names: selectin P
• NCBI Official Symbol: SELP
• NCBI Official Synonym Symbols: CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• NCBI Protein Information: P-selectin
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMRP; GRMP; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_HUMAN

NCBI Description

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; heterophilic cell adhesion; platelet activation; leukocyte adhesion; platelet degranulation; regulation of integrin activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; blood coagulation; inflammatory response; leukocyte tethering or rolling; cell adhesion; leukocyte migration

Disease: Ige Responsiveness, Atopic