Testing Data
(Fig: Western blot analysis on Jurkat using anti-CD62E polyclonal antibody)
CD62E Polyclonal Antibody | anti-CD62E antibody
CD62E (E-selectin, ELAM-1) Antibody
Gene Names
SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
Reactivity
Mouse, Human, Rat
Applications
Western Blot, Immunocytochemistry
Purity
Affinity purified
Synonyms
CD62E; Polyclonal Antibody; CD62E (E-selectin; ELAM-1) Antibody; E-selectin; ELAM-1; anti-CD62E antibody
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of CD62E.
Purity/Purification
Affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
Sequence Length
610
Applicable Applications for anti-CD62E antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB: 1:500
Preservative
0.05% Sodium Azide.
Cellular Localization
Cell Membrane
Positive Control
Jurkat
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Testing Data
(Fig: Western blot analysis on Jurkat using anti-CD62E polyclonal antibody)
Testing Data
(Fig: Western blot analysis on Jurkat using anti-CD62E polyclonal antibody)
Related Product Information for anti-CD62E antibody
CD62E, known as E-selectin, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x. P-selectin has a similar function, but is expressed on the endothelial cell surface within minutes as it is stored within the cell rather than produced on demand.
References
1. Phillips M.L., Nudelman E., et al: "ELAM-1.mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex."; Science 2.0:1.3.-1.3.1.90). 2. Mills A.; "Modelling the carbohydrate recognition domain of human E-selectin."; FEBS Lett. 3.:5-1.(1.93.) Collins T., Williams A., Johnston G.I., et al; "Structure and chromosomal location of the gene for endothelial-leukocyte adhesion molecule 1."; J. Biol. Chem. 2.6:2.66-2.73.1.91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67; 115kDa
NCBI Official Full Name
E-selectin
NCBI Official Synonym Full Names
selectin E
NCBI Official Symbol
SELE
NCBI Official Synonym Symbols
ELAM; ESEL; CD62E; ELAM1; LECAM2
NCBI Protein Information
E-selectin
UniProt Protein Name
E-selectin
UniProt Gene Name
SELE
UniProt Synonym Gene Names
ELAM1; ELAM-1; LECAM2
NCBI Description
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]
Uniprot Description
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Research Articles on CD62E
Similar Products
Product Notes
The CD62E sele (Catalog #AAA857112) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CD62E (E-selectin, ELAM-1) Antibody reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD62E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB: 1:500. Researchers should empirically determine the suitability of the CD62E sele for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD62E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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