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Western Blot (WB) (Western blot analysis of CD53 expression in Jurkat cell lysate.)

Rabbit anti-Human CD53 Polyclonal Antibody | anti-CD53 antibody

Anti-CD53 Rabbit pAb

Gene Names
CD53; MOX44; TSPAN25
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
CD53; Polyclonal Antibody; Anti-CD53 Rabbit pAb; CD53 antigen; MOX44; OX44; RATOX44; Tetraspanin25; Transmembrane glycoprotein; TSPAN25; PRO1340; anti-CD53 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CD53 Antibody detects endogenous levels of total CD53
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
219
Applicable Applications for anti-CD53 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:100-1:500
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human CD53
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of CD53 expression in Jurkat cell lysate.)

Western Blot (WB) (Western blot analysis of CD53 expression in Jurkat cell lysate.)
Related Product Information for anti-CD53 antibody
May be involved in growth regulation in hematopoietic cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
963
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24kDa
NCBI Official Full Name
leukocyte surface antigen CD53 isoform 1
NCBI Official Synonym Full Names
CD53 molecule
NCBI Official Symbol
CD53
NCBI Official Synonym Symbols
MOX44; TSPAN25
NCBI Protein Information
leukocyte surface antigen CD53
UniProt Protein Name
Leukocyte surface antigen CD53
Protein Family
UniProt Gene Name
CD53
UniProt Synonym Gene Names
MOX44; TSPAN25; Tspan-25

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells ().

Research Articles on CD53

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Product Notes

The CD53 cd53 (Catalog #AAA4751305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD53 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:100-1:500 FC/FACS: 1:50. Researchers should empirically determine the suitability of the CD53 cd53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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