Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transportin-3 (TNPO3) Standard Curve.)
Human Transportin-3 (TNPO3) ELISA Kit | TNPO3 elisa kit
Human Transportin-3 (TNPO3) ELISA Kit
Gene Names
TNPO3; IPO12; TRNSR; LGMD1F; LGMDD2; MTR10A; TRN-SR; TRN-SR2
Reactivity
Human
Synonyms
Transportin-3 (TNPO3); Human Transportin-3 (TNPO3) ELISA Kit; TNPO3; IPO12; MTR10A; TRN-SR; TRN-SR2; TRNSR; importin 12; transportin-SR; TNPO3 elisa kit
Reactivity
Human
Specificity
Human Transportin-3 (TNPO3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNPO3. No significant cross-reactivity or interference between Human TNPO3 and analogues was observed.
Sequence Length
957
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transportin-3 (TNPO3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transportin-3 (TNPO3) Standard Curve.)
Related Product Information for TNPO3 elisa kit
Description: This Human Transportin-3 (TNPO3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO3 in samples. An antibody specific for TNPO3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNPO3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins, which are essential precursor-mRNA splicing factors.By yeast 2-hybrid analysis using the RS domain of the E2 protein of human papillomavirus (HPV)-5 as bait, Lai et al. (2000) cloned a TNPO3 splice variant, TRNSR2, from a HeLa cell cDNA library. The deduced 923-amino acid TRNSR2 protein lacks 2 regions of about 30 amino acids each found in the TRNSR protein identified by Kataoka et al. (1999). Northern blot analysis detected ubiquitous expression of a 4.5-kb transcript, with highest expression in testis. Epitope-tagged TRNSR2 localized throughout transfected HeLa cells, but a mutant lacking the N-terminal region colocalized with splicing factor SC35 (SFRS2) in nuclear speckles.
Background: TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins, which are essential precursor-mRNA splicing factors.By yeast 2-hybrid analysis using the RS domain of the E2 protein of human papillomavirus (HPV)-5 as bait, Lai et al. (2000) cloned a TNPO3 splice variant, TRNSR2, from a HeLa cell cDNA library. The deduced 923-amino acid TRNSR2 protein lacks 2 regions of about 30 amino acids each found in the TRNSR protein identified by Kataoka et al. (1999). Northern blot analysis detected ubiquitous expression of a 4.5-kb transcript, with highest expression in testis. Epitope-tagged TRNSR2 localized throughout transfected HeLa cells, but a mutant lacking the N-terminal region colocalized with splicing factor SC35 (SFRS2) in nuclear speckles.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
transportin 3, isoform CRA_a
NCBI Official Synonym Full Names
transportin 3
NCBI Official Symbol
TNPO3
NCBI Official Synonym Symbols
IPO12; TRNSR; LGMD1F; LGMDD2; MTR10A; TRN-SR; TRN-SR2
NCBI Protein Information
transportin-3
UniProt Protein Name
Transportin-3
Protein Family
UniProt Gene Name
TNPO3
UniProt Synonym Gene Names
IPO12; Imp12; TRN-SR
UniProt Entry Name
TNPO3_HUMAN
NCBI Description
The protein encoded by this gene is a nuclear import receptor for serine/arginine-rich (SR) proteins such as the splicing factors SFRS1 and SFRS2. The encoded protein has also been shown to be involved in HIV-1 infection, apparently through interaction with the HIV-1 capsid protein. Two transcript variants encoding different isoforms as well as a noncoding transcript have been found for this gene.[provided by RefSeq, Jul 2010]
Uniprot Description
IPO3: Seems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins RBM4, SFRS1 and SFRS2, by recognizing phosphorylated RS domains. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear import; Nuclear export; Receptor, misc.; Karyopherin
Chromosomal Location of Human Ortholog: 7q32.1
Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleus
Molecular Function: protein binding; receptor activity
Biological Process: splicing factor protein import into nucleus
Disease: Muscular Dystrophy, Limb-girdle, Type 1f
Research Articles on TNPO3
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Product Notes
The Human TNPO3 tnpo3 (Catalog #AAA9713849) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713849 ELISA Kit recognizes Human TNPO3. It is sometimes possible for the material contained within the vial of "Transportin-3 (TNPO3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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