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SDS-Page (SDS-PAGE)

Tetraspanin 25 (TSPAN25) Recombinant Protein | TSPAN25 recombinant protein

Recombinant Tetraspanin 25 (TSPAN25)

Gene Names
CD53; MOX44; TSPAN25
Applications
SDS-Page, Western Blot
Purity
Greater than 80% by SDS-PAGE
Synonyms
Tetraspanin 25 (TSPAN25); Recombinant Tetraspanin 25 (TSPAN25); TSPAN25/Tetraspanin 25; CD53; MOX44; TSPAN25; Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; TSPAN25 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater than 80% by SDS-PAGE
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence
Glu107~Leu219
Applicable Applications for TSPAN25 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Tag
N-terminal His and GST Tag
Expression System
Prokaryotic expression
Organism Species
Human
Isoelectric Point
6.7
Subcellular Location
Membrane
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

SDS-Page

(SDS-PAGE)

SDS-Page (SDS-PAGE)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
963
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,341 Da
NCBI Official Full Name
leukocyte surface antigen CD53
NCBI Official Synonym Full Names
CD53 molecule
NCBI Official Symbol
CD53
NCBI Official Synonym Symbols
MOX44; TSPAN25
NCBI Protein Information
leukocyte surface antigen CD53; CD53 antigen; CD53 glycoprotein; CD53 tetraspan antigen; antigen MOX44 identified by monoclonal antibody MRC-OX44; cell surface antigen; cell surface glycoprotein CD53; tetraspanin-25; transmembrane glycoprotein; tspan-25
UniProt Protein Name
Leukocyte surface antigen CD53
UniProt Gene Name
CD53
UniProt Synonym Gene Names
MOX44; TSPAN25; Tspan-25
UniProt Entry Name
CD53_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CD53: May be involved in growth regulation in hematopoietic cells. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; plasma membrane; integral to membrane; immunological synapse; intercellular junction

Molecular Function: protein binding

Biological Process: signal transduction

Research Articles on TSPAN25

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Product Notes

The TSPAN25 cd53 (Catalog #AAA2125480) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tetraspanin 25 (TSPAN25) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the TSPAN25 cd53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu107~Leu 219. It is sometimes possible for the material contained within the vial of "Tetraspanin 25 (TSPAN25), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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