Rabbit anti-Human CD30/TNFRSF8 Polyclonal Antibody | anti-TNFRSF8 antibody

Anti-CD30/TNFRSF8 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103893

• Gene Names: TNFRSF8; CD30; Ki-1; D1S166E
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CD30/TNFRSF8; Polyclonal Antibody; Anti-CD30/TNFRSF8 Antibody; Rabbit Polyclonal; CD30/TNFRSF8 Antibody; Rabbit PAb; Antigen Affinity Purified; tumor necrosis factor receptor superfamily; member 8; Anti-CD30 Antibody; Anti-D1S166E Antibody; Anti-Ki-1 Antibody; anti-TNFRSF8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD30/TNFRSF8
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TNFRSF8 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000
• Immunogen: Recombinant Human CD30/TNFRSF8 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD30/TNFRSF8 (rh CD30/TNFRSF8). CD30/TNFRSF8 specific IgG was purified by Human CD30/TNFRSF8 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human TNFRSF8 in human hodgkin lymphoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Western Blot (WB)

(Anti-TNFRSF8 rabbit polyclonal antibody at 1:500 dilution.Lane A: A431 Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:64 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-TNFRSF8 antibody

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. CD30 protein is expressed by activated, but not resting, T and B cells. CD30 can regulate proliferation of lymphocytes and may also play an important role in human immunodeficiency virus replication. As a regulator of apoptosis, CD30 protein induces cell death or proliferation, depending on the cell type, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD30 protein expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs), and CD30 is also linked to leukocytes in patients with chronic inflammatory diseases, including lupus erythematosus, asthma, rheumatoid arthritis and atopic dermatitis (AD).

Product Categories/Family for anti-TNFRSF8 antibody

Cancer Drug Targets

References

Rossi FM, et al. (2001) CD30L up-regulates CD30 and IL-4 expression by T cells. FEBS Lett. 508(3): 418-22.
Trovato M, et al. (2001) Expression of CD30 ligand and CD30 receptor in normal thyroid and benign and malignant thyroid nodules. Thyroid. 11(7): 621-8.
Ekstrom ES, et al. (2001) Presence of CD30(+) and CD30L(+) cells in human placenta and soluble CD30 levels in cord blood are independent of maternal atopy. Placenta. 22(4): 372-9.
Tang C, et al. (2008) A novel role of CD30L/CD30 signaling by T-T cell interaction in Th1 response against mycobacterial infection. J Immunol. 181(9): 6316-27.
Sun X, et al. (2008) A critical role of CD30 ligand/CD30 in controlling inflammatory bowel diseases in mice. Gastroenterology. 134(2): 447-58.
Oflazoglu E, et al. (2009) Targeting CD30/CD30L in Oncology and Autoimmune and Inflammatory Diseases.Adv Exp Med Biol. 647: 174-85.

NCBI and Uniprot Product Information

• NCBI GI #: 68348711
• NCBI GeneID: 943
• NCBI Accession #: NP_001234.2
• NCBI GenBank Nucleotide #: NM_001243.3
• UniProt Accession #: P28908; Q6P4D9; B1AN79; Q6GMU3; Q9HCX5
• Molecular Weight: 63,747 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 8 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 8
• NCBI Official Symbol: TNFRSF8
• NCBI Official Synonym Symbols: CD30; Ki-1; D1S166E
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 8; CD30 antigen; Ki-1 antigen; CD30L receptor; OTTHUMP00000001783; OTTHUMP00000221481; cytokine receptor CD30; lymphocyte activation antigen CD30
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 8
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF8
• UniProt Synonym Gene Names: CD30; D1S166E
• UniProt Entry Name: TNR8_HUMAN

Uniprot Description

TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction