Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Proline-rich protein 15 (PRR15) Standard Curve.)
Mouse Proline-Rich Protein 15 (PRR15) ELISA Kit | PRR15 elisa kit
Mouse Proline-Rich Protein 15 (PRR15) ELISA Kit
Gene Names
Prr15; G90; E130201N16Rik
Reactivity
Mouse
Synonyms
Proline-Rich Protein 15 (PRR15); Mouse Proline-Rich Protein 15 (PRR15) ELISA Kit; PRR15 elisa kit
Reactivity
Mouse
Specificity
Mouse Proline-rich protein 15 (PRR15) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRR15. No significant cross-reactivity or interference between Mouse PRR15 and analogues was observed.
Sequence Length
122
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Proline-rich protein 15 (PRR15) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Proline-rich protein 15 (PRR15) Standard Curve.)
Related Product Information for PRR15 elisa kit
Description: This Mouse Proline-rich protein 15 (PRR15) ELISA Kit employs a two-site sandwich ELISA to quantitate PRR15 in samples. An antibody specific for PRR15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRR15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRR15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRR15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proline rich 15 (Prr15), which encodes a protein of unknown function, is expressed almost exclusively in postmitotic cells both during fetal development and in adult tissues, such as the intestinal epithelium and the testis. Prr15/PRR15 expression was consistently observed in mouse gastrointestinal (GI) tumors caused by mutations in the Apc gene, as well as in several advanced stage human CRCs. In contrast, no Prr15 expression was detected in intestinal tumors derived from mice carrying mutations in the Smad3, Smad4, or Cdkn1b genes. These findings, combined with the fact that a majority of sporadic human CRCs carry APC mutations, strongly suggest that the expression of Prr15/PRR15 in mouse and human GI tumors is linked, directly or indirectly, to the absence of the APC protein or, more generally, to the disruption of the Wnt signaling pathway.
Background: Proline rich 15 (Prr15), which encodes a protein of unknown function, is expressed almost exclusively in postmitotic cells both during fetal development and in adult tissues, such as the intestinal epithelium and the testis. Prr15/PRR15 expression was consistently observed in mouse gastrointestinal (GI) tumors caused by mutations in the Apc gene, as well as in several advanced stage human CRCs. In contrast, no Prr15 expression was detected in intestinal tumors derived from mice carrying mutations in the Smad3, Smad4, or Cdkn1b genes. These findings, combined with the fact that a majority of sporadic human CRCs carry APC mutations, strongly suggest that the expression of Prr15/PRR15 in mouse and human GI tumors is linked, directly or indirectly, to the absence of the APC protein or, more generally, to the disruption of the Wnt signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
proline-rich protein 15
NCBI Official Synonym Full Names
proline rich 15
NCBI Official Symbol
Prr15
NCBI Official Synonym Symbols
G90; E130201N16Rik
NCBI Protein Information
proline-rich protein 15
UniProt Protein Name
Proline-rich protein 15
Protein Family
UniProt Gene Name
Prr15
UniProt Synonym Gene Names
G90
UniProt Entry Name
PRR15_MOUSE
Research Articles on PRR15
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Product Notes
The Mouse PRR15 prr15 (Catalog #AAA9718039) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718039 ELISA Kit recognizes Mouse PRR15. It is sometimes possible for the material contained within the vial of "Proline-Rich Protein 15 (PRR15), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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