Goat CD28 Polyclonal Antibody | anti-CD28 antibody

Goat anti-CD28 Antibody

Cat. Num. AAA422554

• Gene Names: CD28; Tp44
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CD28; Polyclonal Antibody; Goat anti-CD28 Antibody; CD28 molecule; MGC138290; Tp44; CD28 antigen; CD28 antigen (Tp44); T-cell-specific surface glycoprotein; CD28 antibody; CD28 molecule antibody; MGC138290 antibody; Tp44 antibody; CD28 antigen antibody; CD28 antigen (Tp44) antibody; T-cell-specific surface glycoprotein antibody; anti-CD28 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SQQLQVYSKTGFNCD
• Sequence Length: 220

• Applicable Applications for anti-CD28 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Approx 48kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence SQQLQVYSKTGFNCD, from the internal region of the protein sequence according to NP_006130.1.
• Epitope: Internal region
• Note: The immunizing peptide corresponds to a part of the extracellular domain.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453611
• NCBI GeneID: 940
• NCBI Accession #: NP_006130.1
• NCBI GenBank Nucleotide #: NM_006139.3
• Molecular Weight: 26,186 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 1
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cell surface; cytosol; external side of plasma membrane; immunological synapse; integral to plasma membrane; plasma membrane

Molecular Function: coreceptor activity; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; protein kinase binding; SH3/SH2 adaptor activity

Biological Process: cell surface receptor linked signal transduction; cytokine biosynthetic process; humoral immune response; negative thymic T cell selection; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of alpha-beta T cell proliferation; positive regulation of inflammatory response to antigenic stimulus; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of isotype switching to IgG isotypes; positive regulation of mitosis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of translation; positive regulation of viral genome replication; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; regulation of regulatory T cell differentiation; regulatory T cell differentiation; T cell costimulation; T cell receptor signaling pathway