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Typical Testing Data/Standard Curve (for reference only)

Porcine Thyrotropin Releasing Hormone (TRH) ELISA Kit | TRH elisa kit

Porcine Thyrotropin Releasing Hormone (TRH) ELISA Kit

Gene Names
TRH; TRF; Pro-TRH
Reactivity
Porcine
Synonyms
Thyrotropin Releasing Hormone (TRH); Porcine Thyrotropin Releasing Hormone (TRH) ELISA Kit; TRH elisa kit
Ordering
For Research Use Only!
Reactivity
Porcine
Specificity
No cross-reaction with other factors.
Sequence Length
242
Samples
Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant TRH concentration.
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
Up to 0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TRH elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine TRH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,404 Da
NCBI Official Full Name
thyrotropin releasing hormone preproprotein
NCBI Official Synonym Full Names
thyrotropin-releasing hormone
NCBI Official Symbol
TRH
NCBI Official Synonym Symbols
TRF; Pro-TRH
NCBI Protein Information
thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor; pro-thyrotropin-releasing hormone
UniProt Protein Name
Pro-thyrotropin-releasing hormone
UniProt Gene Name
TRH
UniProt Synonym Gene Names
Pro-TRH; TRH; TRF
UniProt Entry Name
TRH_HUMAN

NCBI Description

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]

Uniprot Description

TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: extracellular region; plasma membrane; nucleus; secretory granule

Molecular Function: neuropeptide hormone activity; thyrotropin-releasing hormone activity

Biological Process: hormone-mediated signaling; negative regulation of glutamate secretion; eating behavior; positive regulation of insulin secretion; histamine metabolic process; signal transduction; adult walking behavior; response to ethanol; cell-cell signaling; response to glucose stimulus; response to hypoxia; response to cold; response to corticosterone stimulus; positive regulation of gamma-aminobutyric acid secretion

Disease: Thyrotropin-releasing Hormone Deficiency

Research Articles on TRH

Similar Products

Product Notes

The Porcine TRH trh (Catalog #AAA265712) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA265712 ELISA Kit recognizes Porcine TRH. It is sometimes possible for the material contained within the vial of "Thyrotropin Releasing Hormone (TRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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