Western Blot (WB)
(Anti-CD2 antibody, MBS175165, Western blottingLane 1: Rat Spleen Cell LysateLane 2: NIH/3T3 Cell Lysate )
Rabbit anti-Mouse, Rat CD2 Polyclonal Antibody | anti-CD2 antibody
Anti-CD2 antibody
Gene Names
Cd2; Ly37; LFA-2; Ly-37
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
CD2; Polyclonal Antibody; Anti-CD2 antibody; T-cell surface antigen CD2; CD2 molecule; CD 2 antibody; CD2 antibody; CD2 antigen(p50); sheep red blood cell receptor antibody; CD2 antigen antibody; CD2 molecule antibody; CD2_HUMAN antibody; Erythrocyte receptor antibody; FLJ46032 antibody; LFA-2 antibody; LFA-3 receptor antibody; LFA2 antibody; LFA3 receptor antibody; Ly-37 antibody; Lymphocyte function antigen 2 antibody; lymphocyte-function antigen-2 antibody; OTTHUMP00000024366 antibody; Rosette receptor antibody; SRBC antibody; T cell surface antigen CD2 antibody; T-cell surface antigen CD2 antibody; T-cell surface antigen T11/Leu-5 antibody; T-lymphocyte surface CD2 antigen antibody; T11 antibody; anti-CD2 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
344
Applicable Applications for anti-CD2 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CD2 (224-242aa FIFCICKRRKRNRRRKDEE), different from the related rat sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-CD2 antibody, MBS175165, Western blottingLane 1: Rat Spleen Cell LysateLane 2: NIH/3T3 Cell Lysate )
Western Blot (WB)
(Anti-CD2 antibody, MBS175165, Western blottingLane 1: Rat Spleen Cell LysateLane 2: NIH/3T3 Cell Lysate )
Related Product Information for anti-CD2 antibody
Description: Rabbit IgG polyclonal antibody for T-cell surface antigen CD2(CD2) detection. Tested with WB in Mouse, Rat.
Background: CD2(cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer(NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al.(1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3(LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.
Background: CD2(cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer(NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al.(1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3(LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,415 Da
NCBI Official Full Name
T-cell surface antigen CD2
NCBI Official Synonym Full Names
CD2 antigen
NCBI Official Symbol
Cd2
NCBI Official Synonym Symbols
Ly37; LFA-2; Ly-37
NCBI Protein Information
T-cell surface antigen CD2; LFA-3 receptor; lymphocyte antigen 37; T-cell surface antigen T11/Leu-5
UniProt Protein Name
T-cell surface antigen CD2
Protein Family
UniProt Gene Name
Cd2
UniProt Synonym Gene Names
Ly-37; Ly-37
UniProt Entry Name
CD2_MOUSE
Uniprot Description
CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.
Protein type: Membrane protein, integral; Apoptosis; Cell surface
Cellular Component: internal side of plasma membrane; cell surface; protein complex; anchored to plasma membrane; membrane; integral to membrane; extracellular region; intercellular junction; lipid raft; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; protein self-association; receptor activity; glycoprotein binding; receptor tyrosine kinase binding; protein kinase binding; antigen binding; receptor binding
Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; T cell activation; cell adhesion; positive regulation of tumor necrosis factor production
Research Articles on CD2
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Product Notes
The CD2 cd2 (Catalog #AAA175165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD2 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CD2 cd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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