Western Blot (WB)
(Western analysis of recombinant human sCD31 using a rabbit anti-human CD31 polyclonal antibody.)
Rabbit anti-Human CD31/PECAM-1 Polyclonal Antibody | anti-CD31/PECAM-1 antibody
Rabbit Anti-Human CD31/PECAM-1
IF:Use at 2-10 ?g/ml
IF/IHC: Use at 1-5 ?g/ml
FC: Use at 2-10?g/ml
Western Blot (WB)
(Western analysis of recombinant human sCD31 using a rabbit anti-human CD31 polyclonal antibody.)
Western Blot (WB)
(Western analysis of recombinant human sCD31 using a rabbit anti-human CD31 polyclonal antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of human CD31 in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal firbroblast (NHDF) with a polyclonal rabbit anti-human CD31 antibody. Conjugated secondary antibody: donkey anti rabbit ALEXA Flour 488 (1:600) [Dianova].)
Immunofluorescence (IF)
(Immunofluorescence staining of human CD31 in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal firbroblast (NHDF) with a polyclonal rabbit anti-human CD31 antibody. Conjugated secondary antibody: donkey anti rabbit ALEXA Flour 488 (1:600) [Dianova].)
Immunofluorescence (IF)
(Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human CD31 (5ug/ml) and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.)
Immunofluorescence (IF)
(Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human CD31 (5ug/ml) and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.)
Flow Cytommetry (FC)
(FACS analysis of CD31 expression in human primary umbilical vein endothelial cells.)
Flow Cytommetry (FC)
(FACS analysis of CD31 expression in human primary umbilical vein endothelial cells.)
Immunofluorescence (IF)
(Immunofluorescence staining of human CD31in primary human dermal lymphatic endothelial cells (HDLEC) and primary human umbilical vein endothelial cells (HUVEC) with a polyclonal rabbit anti-human CD31 antibody [MBS692250]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].)
Immunofluorescence (IF)
(Immunofluorescence staining of human CD31in primary human dermal lymphatic endothelial cells (HDLEC) and primary human umbilical vein endothelial cells (HUVEC) with a polyclonal rabbit anti-human CD31 antibody [MBS692250]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].)
NCBI and Uniprot Product Information
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: extracellular space; plasma membrane; platelet alpha granule membrane
Molecular Function: protein binding
Biological Process: cell recognition; diapedesis; extracellular matrix organization and biogenesis; leukocyte migration; phagocytosis; platelet degranulation; signal transduction
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Product Notes
The CD31/PECAM-1 pecam1 (Catalog #AAA692250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human CD31/PECAM-1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD31/PECAM-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytommetry (FC). WB: Use at 1-5 ?g/ml IF:Use at 2-10 ?g/ml IF/IHC: Use at 1-5 ?g/ml FC: Use at 2-10?g/ml. Researchers should empirically determine the suitability of the CD31/PECAM-1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31/PECAM-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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