Western Blot (WB)
(Western blot analysis of CD14 expression in rat brain extract (lane 1) and mouse liver extract (lane 2). CD14 at 50KD was detected using rabbit anti-CD14 Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
CD14 Polyclonal Antibody | anti-CD14 antibody
Anti-CD14 Antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat
Predicted Species: Hu
Immunohistochemistry (Paraffin-embedded Section) (IHC-P):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Ms, Rat
Antigen Retrieval: By Heat
Flow Cytometry (FC): Concentration: 1-3ug/1x106 cells
Tested Species: Hu
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin section in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Western blot analysis of CD14 expression in rat brain extract (lane 1) and mouse liver extract (lane 2). CD14 at 50KD was detected using rabbit anti-CD14 Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CD14 expression in rat brain extract (lane 1) and mouse liver extract (lane 2). CD14 at 50KD was detected using rabbit anti-CD14 Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(CD14 was detected in paraffin-embedded sections of mouse lymphaden tissues using rabbit anti- CD14 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD14 was detected in paraffin-embedded sections of mouse lymphaden tissues using rabbit anti- CD14 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD14 was detected in paraffin-embedded sections of rat lymphaden tissues using rabbit anti- CD14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD14 was detected in paraffin-embedded sections of rat lymphaden tissues using rabbit anti- CD14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD14 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- CD14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD14 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- CD14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Uniprot Description
CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: anchored to external side of plasma membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; lipid raft; lipopolysaccharide receptor complex; plasma membrane
Molecular Function: lipopolysaccharide binding; opsonin receptor activity; peptidoglycan receptor activity; protein binding
Biological Process: apoptosis; cell surface receptor linked signal transduction; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of MyD88-independent toll-like receptor signaling pathway; phagocytosis; positive regulation of cytokine secretion; positive regulation of endocytosis; positive regulation of interferon type I production; positive regulation of interferon-gamma production; positive regulation of tumor necrosis factor production; receptor-mediated endocytosis; response to electrical stimulus; response to ethanol; response to heat; response to magnesium ion; toll-like receptor 4 signaling pathway
Research Articles on CD14
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Product Notes
The CD14 cd14 (Catalog #AAA177790) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD14 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot (WB): Concentration: 0.1-0.5ug/ml Tested Species: Ms, Rat Predicted Species: Hu Immunohistochemistry (Paraffin-embedded Section) (IHC-P): Concentration: 0.5-1ug/ml Tested Species: Hu, Ms, Rat Antigen Retrieval: By Heat Flow Cytometry (FC): Concentration: 1-3ug/1x106 cells Tested Species: Hu Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin section in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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