Rabbit anti-Human, Mouse VAV3 Polyclonal Antibody | anti-VAV3 antibody

Rabbit Anti VAV3

Cat. Num. AAA225585

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified IgG was prepared from whole serum by affinity chromatography.
• Synonyms: VAV3; Polyclonal Antibody; Rabbit Anti VAV3; VAV3 antibody; anti-VAV3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified IgG was prepared from whole serum by affinity chromatography.
• Form/Format: Liquid; Phosphate Buffered Saline
• Sequence Length: 287

• Applicable Applications for anti-VAV3 antibody: Western Blot (WB)
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 560-680 of human VAV3.
• Preservative Stabilizers: 0.02% Sodium Azide 50% Glycerol
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.; Should this product contain a precipitate we recommend microcentrifugation before use.

Western Blot (WB)

(Western blot analysis of Jurkat cell lysate and mouse brain, lung and spleen tissue lysates using Rabbit anti VAV3 antibody at a 1/1000 dilution. 3% non-fat dry milk was used for blocking)

Related Product Information for anti-VAV3 antibody

Rabbit anti VAV3 antibody recognizes guanine nucleotide exchange factor, also known as VAV-3. It belongs to the VAV family of proteins; the main function of VAV proteins is to work as guanosine nucleotide exchange factors (GEFs) for members of the Rho GTPase family (Bustelo 2014). VAV3 was initially detected in expressed sequence tag (EST) libraries (Movilla and Bustelo 1999). It mediates receptor protein tyrosine kinase signaling (Zeng et al. 2000). Protein overexpression of VAV3 has been reported in several types of human cancer, including breast cancer (Lee et al. 2008) and prostate cancer (Dong et al. 2006).

NCBI and Uniprot Product Information

• NCBI GI #: 120432042
• NCBI GeneID: 10451
• NCBI Accession #: NP_001073343.1
• NCBI GenBank Nucleotide #: NM_001079874.1
• UniProt Accession #: Q9UKW4
• NCBI Official Full Name: guanine nucleotide exchange factor VAV3 isoform 2
• NCBI Official Synonym Full Names: vav guanine nucleotide exchange factor 3
• NCBI Official Symbol: VAV3
• NCBI Protein Information: guanine nucleotide exchange factor VAV3
• UniProt Protein Name: Guanine nucleotide exchange factor VAV3
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: VAV3
• UniProt Synonym Gene Names: VAV-3
• UniProt Entry Name: VAV3_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

VAV3: a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by activated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. Interacts with the PH domain of APS. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation. Down-regulated by EGF and TGF-beta. Four isoforms of the human protein are produced by alternative promoter. Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Adaptor/scaffold; Actin-binding; GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; metal ion binding; Rac guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTPase activator activity; epidermal growth factor receptor binding

Biological Process: response to drug; lamellipodium biogenesis; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; axon guidance; positive regulation of cell adhesion; positive regulation of signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of phosphoinositide 3-kinase activity; vesicle fusion; regulation of small GTPase mediated signal transduction; B cell receptor signaling pathway; small GTPase mediated signal transduction; ephrin receptor signaling pathway; positive regulation of B cell proliferation; innate immune response; angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus