Western Blot (WB)
((1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CD14 Polyclonal Antibody | anti-CD14 antibody
Goat anti-CD14 Antibody
Immunohistochemistry: In paraffin embedded Human Spleen shows strong clustered staining in some splenocytes. Recommended concentration, 4-6ug/ml.
Western Blot: Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun.2001 Jun;69(6): 3772-81; PMID: 11349042). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Uniprot Description
CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: anchored to external side of plasma membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; lipid raft; lipopolysaccharide receptor complex; plasma membrane
Molecular Function: lipopolysaccharide binding; opsonin receptor activity; peptidoglycan receptor activity; protein binding
Biological Process: apoptosis; cell surface receptor linked signal transduction; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of MyD88-independent toll-like receptor signaling pathway; phagocytosis; positive regulation of cytokine secretion; positive regulation of endocytosis; positive regulation of interferon type I production; positive regulation of interferon-gamma production; positive regulation of tumor necrosis factor production; receptor-mediated endocytosis; response to electrical stimulus; response to ethanol; response to heat; response to magnesium ion; toll-like receptor 4 signaling pathway
Research Articles on CD14
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Product Notes
The CD14 cd14 (Catalog #AAA421195) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CD14 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Spleen shows strong clustered staining in some splenocytes. Recommended concentration, 4-6ug/ml. Western Blot: Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun.2001 Jun;69(6): 3772-81; PMID: 11349042). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRVDADADPR QYAD. It is sometimes possible for the material contained within the vial of "CD14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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