Rabbit CD11c Polyclonal Antibody | anti-CD11c antibody

RABBIT ANTI HUMAN CD11c

Cat. Num. AAA221513

• Gene Names: ITGAX; CD11C; SLEB6
• Applications: Immunohistochemistry
• Synonyms: CD11c; Polyclonal Antibody; RABBIT ANTI HUMAN CD11c; anti-CD11c antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects human CD11c, also known as integrin alpha X (ITGAX). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. CD11c combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). CD11c is 150kD in size, and is expressed predominantly on tissue macrophages and dendritic cells. ; ; CD11c binds a myriad of ligands including cell adhesion molecules (e.g. ICAM-1, ICAM-4), bacterial cell wall components (LPS), complement protein (iC3b), and matrix proteins such as fibrogen and collagen. In light of this, CD11c has a number of functions including phagocytosis, cell migration, and cytokine production by macrophages/monocytes, as well as induction of T cell proliferation by Langerhans cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1163

• Applicable Applications for anti-CD11c antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 5; Maximum Dilution: 15ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide: Internal domain of human CD11c, conjugated to KLH.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to CD11c were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human tonsil with Rabbit anti Human CD11c (MBS221513))

Related Product Information for anti-CD11c antibody

MBS221513 detects human CD11c, also known as integrin alpha X (ITGAX). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. CD11c combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). CD11c is 150kD in size, and is expressed predominantly on tissue macrophages and dendritic cells. CD11c binds a myriad of ligands including cell adhesion molecules (e.g. ICAM-1, ICAM-4), bacterial cell wall components (LPS), complement protein (iC3b), and matrix proteins such as fibrogen and collagen. In light of this, CD11c has a number of functions including phagocytosis, cell migration, and cytokine production by macrophages/monocytes, as well as induction of T cell proliferation by Langerhans cells.

NCBI and Uniprot Product Information

• NCBI GI #: 34452173
• NCBI GeneID: 3687
• NCBI Accession #: NP_000878.2
• NCBI GenBank Nucleotide #: NM_000887.4
• UniProt Accession #: P20702; Q8IVA6
• Molecular Weight: 127,829 Da
• NCBI Official Full Name: integrin alpha-X isoform 2
• NCBI Official Synonym Full Names: integrin, alpha X (complement component 3 receptor 4 subunit)
• NCBI Official Symbol: ITGAX
• NCBI Official Synonym Symbols: CD11C; SLEB6
• NCBI Protein Information: integrin alpha-X; CD11 antigen-like family member C; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain
• UniProt Protein Name: Integrin alpha-X
• UniProt Gene Name: ITGAX
• UniProt Synonym Gene Names: CD11C
• UniProt Entry Name: ITAX_HUMAN

NCBI Description

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; integrin complex

Molecular Function: protein binding; metal ion binding; receptor activity

Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; organ morphogenesis; extracellular matrix organization and biogenesis; cell adhesion; blood coagulation; leukocyte migration

Disease: Systemic Lupus Erythematosus