Rabbit anti-Human CCNE1 Polyclonal Antibody | anti-Ccne1 antibody

CCNE1, CT (G1/S-specific Cyclin-E1, CCNE)

Cat. Num. AAA6009724

• Gene Names: Ccne1; Ccne; CYCLE
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CCNE1; Polyclonal Antibody; CT (G1/S-specific Cyclin-E1; CCNE); Anti -CCNE1; anti-Ccne1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Cyclin E1. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.

• Applicable Applications for anti-Ccne1 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human Cyclin E1 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Breast carcinoma stained with Cyclin E antibody)

Immunohistochemistry (IHC)

(Human Placenta (formalin-fixed, paraffin-embedded) stained with CCNE1 antibody at 10ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Testis (formalin-fixed, paraffin-embedded) stained with CCNE1 antibody at 10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-Ccne1 antibody

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Product Categories/Family for anti-Ccne1 antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 171847100
• NCBI GeneID: 25729
• UniProt Accession #: P39949; O09138
• Molecular Weight: 47,482 Da
• NCBI Official Full Name: Ccne1 protein
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: Ccne1
• NCBI Official Synonym Symbols: Ccne; CYCLE
• NCBI Protein Information: G1/S-specific cyclin-E1; cyclin E
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: Ccne1
• UniProt Synonym Gene Names: Ccne
• UniProt Entry Name: CCNE1_RAT

NCBI Description

a cyclin-like protein; may be involved in growth stimulation [RGD, Feb 2006]

Uniprot Description

Function: Essential for the control of the cell cycle at the G1/S (start) transition.

Subunit structure: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation

By similarity.

Subcellular location: Nucleus

By similarity.

Post-translational modification: Phosphorylation of both Thr-396 by GSK3 and Ser-400 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7

By similarity.Ubiquitinated by UHRF2; appears to occur independently of phosphorylation

By similarity.

Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily.

Sequence caution: The sequence BAA03116.1 differs from that shown. Reason: Frameshift at position 15.