Western Blot (WB)
(Figure 1. Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody (MBS1750677).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate,Lane 2: rat lung tissue lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat stomach tissue lysate,Lane 5: rat PC-12 whole Cell lysate,Lane 6: mouse thymus tissue lysate,Lane 7: mouse NIH3T3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD. )
Rabbit Ccl19/MIP-3 beta Polyclonal Antibody | anti-Ccl19 antibody
Anti-Ccl19/MIP-3 beta Picoband Antibody
Reactivity
Mouse, RatNo cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
Ccl19/MIP-3 beta; Polyclonal Antibody; Anti-Ccl19/MIP-3 beta Picoband Antibody; C-C motif chemokine; Ccl19; Ccl19_predicted; rCG_54984; anti-Ccl19 antibody
Host
Rabbit
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified
Form/Format
Lyophilized
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (varies by lot)
Sequence Length
108
Applicable Applications for anti-Ccl19 antibody
Western Blot (WB)
Application Notes
WB: 0.1-0.5mug/ml
Immunogen
E Coli-derived rat Ccl19/MIP-3 beta recombinant protein (Position: G26-S108). Rat Ccl19/MIP-3 beta shares 79.5% and 89.2% amino acid (aa) sequence identity with human and mouse Ccl19/MIP-3 beta, respectively.
Subcellular Localization
Secreted.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody (MBS1750677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysate,Lane 2: rat lung tissue lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat stomach tissue lysate,Lane 5: rat PC-12 whole Cell lysate,Lane 6: mouse thymus tissue lysate,Lane 7: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody (MBS1750677).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate,Lane 2: rat lung tissue lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat stomach tissue lysate,Lane 5: rat PC-12 whole Cell lysate,Lane 6: mouse thymus tissue lysate,Lane 7: mouse NIH3T3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD. )
Related Product Information for anti-Ccl19 antibody
Chemokine (C-C motif) ligand 19 (CCL19) is a protein that in humans is encoded by the CCL19 gene. This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11763 MW
NCBI Official Full Name
C-C motif chemokine 19
NCBI Official Synonym Full Names
C-C motif chemokine ligand 19
NCBI Official Symbol
Ccl19
NCBI Protein Information
C-C motif chemokine 19
UniProt Protein Name
C-C motif chemokine
Protein Family
UniProt Gene Name
Ccl19
Research Articles on Ccl19
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Product Notes
The Ccl19 ccl19 (Catalog #AAA1750677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ccl19/MIP-3 beta Picoband Antibody reacts with Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Ccl19/MIP-3 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.1-0.5mug/ml. Researchers should empirically determine the suitability of the Ccl19 ccl19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ccl19/MIP-3 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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