Immunohistochemistry (IHC)
(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human CCDC88A Polyclonal Antibody | anti-CCDC88A antibody
CCDC88A, CT (Girdin, Akt Phosphorylation Enhancer, APE, Coiled-coil Domain-containing Protein 88A, G alpha-interacting Vesicle-associated Protein, GIV, Girders of Actin Filament, Hook-related Protein 1, HkRP1, APE, GRDN, KIAA1212)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1-2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunohistochemistry (IHC)
(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2ug/ml.)
Western Blot (WB)
(Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
Function: Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation
By similarity. Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Ref.1 Ref.2
Subunit structure: Interacts (via C-terminus) with DISC1; the interaction is direct. Interacts with AKT proteins; the interaction is inhibited in presence of DISC1
By similarity. Homodimer. The non-phosphorylated form interacts with phosphatidylinositol 4-phosphate [PI4P] and weakly with phosphatidylinositol 3-phosphate [PI3P]. Interacts with microtubules. Interacts with actin through its C-terminal domain. Interacts with the C-terminus of AKT1/PKB. Ref.1
Subcellular location: Membrane. Cell membrane. Cytoplasm › cytosol. Cytoplasmic vesicle. Cell projection › lamellipodium. Note: Localizes to the cell membrane through interaction with phosphoinositides. Ref.1 Ref.2 Ref.7
Tissue specificity: Expressed ubiquitously. Ref.1
Post-translational modification: Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.
Sequence similarities: Belongs to the CCDC88 family.
Sequence caution: The sequence AAY14932.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAD97945.1 differs from that shown. Reason: Intron retention at the C-terminus.The sequence CAI46020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on CCDC88A
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Product Notes
The CCDC88A ccdc88a (Catalog #AAA647455) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCDC88A, CT (Girdin, Akt Phosphorylation Enhancer, APE, Coiled-coil Domain-containing Protein 88A, G alpha-interacting Vesicle-associated Protein, GIV, Girders of Actin Filament, Hook-related Protein 1, HkRP1, APE, GRDN, KIAA1212) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC88A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the CCDC88A ccdc88a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC88A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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