anti-Human Caspase 9, p35 Polyclonal Antibody | anti-CASP9 antibody
Anti - Caspase 9, p35 (ICE-LAP6, Mch6, Apaf 3)
Gene Names
CASP9; MCH6; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Caspase 9; p35; Polyclonal Antibody; Anti - Caspase 9; p35 (ICE-LAP6; Mch6; Apaf 3); anti-CASP9 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human control peptide is 68% conserved in rat, 65% in mouse, 64% in xenopus, and 62% in chicken caspase-9. No significant sequence homology is detected with other caspases. Since control peptide Is located at the C-terminus of p35 subunit, the antibodies will not detect the p10 subunit.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Concentration
~1mg/ml (varies by lot)
Sequence Length
416
Applicable Applications for anti-CASP9 antibody
ELISA, Western Blot
Application Notes
Suitable for use in Western Blot and ELISA. Other applications not tested.
Dilution: Western Blotting (1-10ug/ml for affinity pure antibody using ECL technique). Caspase -9 p35 subunit is ~35 Kd.
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure).
Optimal dilution determined by the researcher.
Dilution: Western Blotting (1-10ug/ml for affinity pure antibody using ECL technique). Caspase -9 p35 subunit is ~35 Kd.
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure).
Optimal dilution determined by the researcher.
Immunogen
25-aa peptide of human Caspase-9~CT of p35 subunit
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CASP9 antibody
Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc. Apoptosis is driven by specialized proteases known as caspases. After the initial discovery of the first mammalian caspase 1 or ICE (interleukin 1 beta converting enzyme), a growing family of caspases 1-14 have been cloned and characterized. Caspases are synthesized as inactive zymogen or proenzyme forms (30-55kD), which upon apoptotic stimulation are proteolytically processed (self or by other proteins) in a sequential manner into their active heterotetrameric forms. The processed form consists of large subunit (17-20kD) and a small (10-12kD) subunits, which may associate to form an active enzyme. Functionally active caspases initiate a proteolytic cascade, capable of cleaving and activating numerous cellular targets including PARP, G4-GDI, DFF, MEKK, etc. On a functional basis, two categories of caspases have been defined: the initiator caspases (caspases-8, -9, and -10) are activated in the earlier phases of apoptosis, whereas the executioner caspases (caspases-3, -6, and -7) are activated by initiator caspases and are responsible for dismantling cellular components. Caspases are widely distributed in various tissues and cells.
Product Categories/Family for anti-CASP9 antibody
References
• 1. Duan H et al (196) JBC 271, 16720; Srinivasula SM et al (1996) JBC 271, 27099; Angelastro JM et al (2001) JBC 276, 12190
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
46,281 Da
NCBI Official Full Name
Caspase-9
NCBI Official Synonym Full Names
caspase 9, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP9
NCBI Official Synonym Symbols
MCH6; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
NCBI Protein Information
caspase-9; OTTHUMP00000002322; OTTHUMP00000002323; OTTHUMP00000002324; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3
UniProt Protein Name
Caspase-9
Protein Family
UniProt Gene Name
CASP9
UniProt Synonym Gene Names
MCH6
UniProt Entry Name
CASP9_HUMAN
Similar Products
Product Notes
The CASP9 casp9 (Catalog #AAA610075) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Caspase 9, p35 (ICE-LAP6, Mch6, Apaf 3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 9, p35 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in Western Blot and ELISA. Other applications not tested. Dilution: Western Blotting (1-10ug/ml for affinity pure antibody using ECL technique). Caspase -9 p35 subunit is ~35 Kd. ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure). Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the CASP9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 9, p35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
