Immunoblotting (IB)
(Immunoblotting at a dilution of 1:1,000 - 1:5,000.)
Goat 6 His HRP Polyclonal Antibody
6-His Affinity Purified Antibody, HRP Conjugated
Applications
ELISA, Immunoblot, Western Blot, Immunocytochemistry
Synonyms
6 His HRP; Polyclonal Antibody; 6-His Affinity Purified Antibody; HRP Conjugated; anti-6 His HRP antibody
Host
Goat
Clonality
Polyclonal
Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Form/Format
Conjugated to horseradish peroxidase (HRP); In Phosphate-Buffered Saline (PBS), pH 7.2, 0.2% BSA, 0.05% Pro-Clean 400.
Molar enzyme/antibody ratio is 4:1.
Molar enzyme/antibody ratio is 4:1.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-6 His HRP antibody
ELISA (EIA), Immunoblotting (IB), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:10,000-1:100,000
ICC: 1:200-1:500
Optimal dilutions/concentrations should be determined by the end user.
Other applications not yet tested, however should not be excluded.
ICC: 1:200-1:500
Optimal dilutions/concentrations should be determined by the end user.
Other applications not yet tested, however should not be excluded.
Antigen
6-His (HHHHHH) conjugated to KLH.
Dilution Instructions
Dilute in PBS or medium that is identical to that used in the assay system.
Note
For in vitro investigational use only.
Preparation and Storage
Store at 2-8°C for one year.
Immunoblotting (IB)
(Immunoblotting at a dilution of 1:1,000 - 1:5,000.)
Immunoblotting (IB)
(Immunoblotting at a dilution of 1:1,000 - 1:5,000.)
Product Categories/Family for anti-6 His HRP antibody
Similar Products
Product Notes
The 6 His HRP (Catalog #AAA194007) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 6 His HRP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoblotting (IB), Immunocytochemistry (ICC). ELISA: 1:10,000-1:100,000 ICC: 1:200-1:500 Optimal dilutions/concentrations should be determined by the end user. Other applications not yet tested, however should not be excluded. Researchers should empirically determine the suitability of the 6 His HRP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "6 His HRP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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