Rabbit anti-Mouse, Rat STEAP1 Polyclonal Antibody | anti-STEAP1 antibody

RABBIT ANTI STEAP1 (C-TERMINAL)

Cat. Num. AAA222893

• Gene Names: STEAP1; STEAP; PRSS24
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: STEAP1; Polyclonal Antibody; RABBIT ANTI STEAP1 (C-TERMINAL); anti-STEAP1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises STEAP1 (six transmembrane epithelial antigen of the prostate), a 39kD protein which is a member of a family of cell surface serpentine transmembrane antigens of the prostate. ; ; STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 339

• Applicable Applications for anti-STEAP1 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 40kDa in human spleen cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; Preparation
• Immunogen: An 18 amino acid peptide located near the c-terminal region of human STEAP1.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human spleen lysate probed with Rabbit ati Human STEAP1 (MBS222893) at 1(A) and 2(B) ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STEAP1 (MBS222893) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-STEAP1 antibody

MBS222893 recognises STEAP1 (six transmembrane epithelial antigen of the prostate), a 39kD protein which is a member of a family of cell surface serpentine transmembrane antigens of the prostate. STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 9558759
• NCBI GeneID: 26872
• NCBI Accession #: NP_036581.1
• NCBI GenBank Nucleotide #: NM_012449.2
• UniProt Accession #: Q9UHE8; O95034; A4D1E0
• Molecular Weight: 39,851 Da
• NCBI Official Full Name: metalloreductase STEAP1
• NCBI Official Synonym Full Names: six transmembrane epithelial antigen of the prostate 1
• NCBI Official Symbol: STEAP1
• NCBI Official Synonym Symbols: STEAP; PRSS24
• NCBI Protein Information: metalloreductase STEAP1; six-transmembrane epithelial antigen of prostate 1
• UniProt Protein Name: Metalloreductase STEAP1
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP1
• UniProt Synonym Gene Names: PRSS24; STEAP
• UniProt Entry Name: STEA1_HUMAN

NCBI Description

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]

Uniprot Description

STEAP1: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; EC 1.16.1.-

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: membrane; integral to plasma membrane; plasma membrane; endosome membrane; intercellular junction

Molecular Function: transporter activity; channel activity; metal ion binding; oxidoreductase activity

Biological Process: iron ion homeostasis; ion transport; transmembrane transport