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Western Blot (WB)
(Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1, 000 dilution.)
Rabbit Alcohol Dehydrogenase Monoclonal Antibody | anti-ADH antibody
Alcohol Dehydrogenase Antibody
Gene Names
ADH1A; ADH1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
ProA affinity purified
Synonyms
Alcohol Dehydrogenase; Monoclonal Antibody; Alcohol Dehydrogenase Antibody; ADH alpha subunit antibody; ADH antibody; ADH1 antibody; ADH1A antibody; ADH1A_HUMAN antibody; Alcohol dehydrogenase 1 (class I); alpha polypeptide antibody; Alcohol dehydrogenase 1 antibody; Alcohol dehydrogenase 1A (class I); Alcohol dehydrogenase 1A antibody; Alcohol dehydrogenase subunit alpha antibody; Aldehyde reductase antibody; anti-ADH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG82-31
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ADH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human Alcohol Dehydrogenase aa 1-200.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1, 000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-ADH antibody
The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster. The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract.
Product Categories/Family for anti-ADH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
375
NCBI Official Full Name
alcohol dehydrogenase 1A
NCBI Official Synonym Full Names
alcohol dehydrogenase 1A (class I), alpha polypeptide
NCBI Official Symbol
ADH1A
NCBI Official Synonym Symbols
ADH1
NCBI Protein Information
alcohol dehydrogenase 1A; ADH, alpha subunit; aldehyde reductase; alcohol dehydrogenase subunit alpha; alcohol dehydrogenase 1 (class I), alpha polypeptide
UniProt Protein Name
Alcohol dehydrogenase 1A
Protein Family
UniProt Gene Name
ADH1A
UniProt Synonym Gene Names
ADH1
UniProt Entry Name
ADH1A_HUMAN
Similar Products
Product Notes
The ADH adh1a (Catalog #AAA9436669) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Alcohol Dehydrogenase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alcohol Dehydrogenase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:100. Researchers should empirically determine the suitability of the ADH adh1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alcohol Dehydrogenase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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