Rabbit Caspase 12 Polyclonal Antibody | anti-Casp12 antibody

Caspase 12 (NT) (MaxLight 750)

Cat. Num. AAA6467601

• Reactivity: Mouse, Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Caspase 12; Polyclonal Antibody; Caspase 12 (NT) (MaxLight 750); anti-Casp12 antibody

• Host: Rabbit
• Reactivity: Mouse, Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes an epitope within the N-terminal region of mouse caspase 12. Species Crossreactivity: Human and rat.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight750.

• Applicable Applications for anti-Casp12 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 10ug/ml; Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0, is recommended.; WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids 2-17 of murine caspase 12.
• Conjugate: MaxLight750
• Positive Control: Heart tissue (IHC)
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Caspase 12-C2087-73 staining of human heart tissue with anti-Caspase-12 (NT) at 10 ug/ml. Formalin fixed paraffin processed tissue.)

Product Categories/Family for anti-Casp12 antibody

Antibodies; Apoptosis; Caspase

References

1. Van de Croen, M., et al., FEBS Lett. 403: 61-69 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 2094806
• NCBI GeneID: 12364
• UniProt Accession #: O08736; Q3TT82
• Molecular Weight: 47,854 Da
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12; CASP-12
• UniProt Protein Name: Caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_MOUSE

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Protease

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; cytosol; nucleus

Molecular Function: peptidase activity; protein binding; protease binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; protein autoprocessing; unfolded protein response; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; proteolysis