Rabbit anti-Mouse CASP3 Polyclonal Antibody | anti-CASP3 antibody

CASP3, Phosphorylated (S12) (Casp3, Cpp32, Caspase-3, Apopain, Cysteine protease CPP32, LICE, Protein Yama, SREBP cleavage activity 1, Caspase-3 subunit p17, Caspase-3 subunit p12) (Azide free) (HRP)

Cat. Num. AAA6280907

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; Apopain; Caspase-3; A830040C14Rik
• Reactivity: Mouse
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CASP3; Polyclonal Antibody; Phosphorylated (S12) (Casp3; Cpp32; Caspase-3; Apopain; Cysteine protease CPP32; LICE; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12) (Azide free) (HRP); Casp3; Caspase-3 subunit p12; anti-CASP3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CASP3 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-mouse CASP3-S12 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of mouse CASP3.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-mouse CASP3-S12 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-CASP3 antibody

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].

Product Categories/Family for anti-CASP3 antibody

Antibodies; Apoptosis; Caspase

References

Srikanth, C.V., et al. Science 330(6002):390-393(2010). Li, F., et al. Cell Stem Cell 7(4):508-520(2010). Wang, L., et al. J. Neurosci. 30(39):13201-13210(2010). Gascon, E., et al. J. Neurosci. 30(37):12414-12423(2010). Bohsali, A., et al. BMC Microbiol. 10, 237 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6753284
• NCBI GeneID: 12367
• NCBI Accession #: NP_033940.1
• NCBI GenBank Nucleotide #: NM_009810.2
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• Molecular Weight: 31,475 Da
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; Apopain; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3; SCA-1; CASP-3; CPP-32; protein Yama; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.