Rabbit anti-Human, Mouse CASP12 Polyclonal Antibody | anti-CASP12 antibody

CASP12 Antibody

Cat. Num. AAA7050494

• Gene Names: CASP12; CASP-12; CASP12P1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: CASP12; Polyclonal Antibody; CASP12 Antibody; Inactive caspase-12; CASP-12; UNQ9415/PRO34398; anti-CASP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 341

• Applicable Applications for anti-CASP12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Inactive caspase-12 protein (61-282AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: CASP12 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39,20,30 KDaObserved band size: 39 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7050494 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7050494 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-CASP12 antibody

Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.

NCBI and Uniprot Product Information

• NCBI GI #: 617418490
• NCBI GeneID: 100506742
• NCBI Accession #: NP_001177945.2
• NCBI GenBank Nucleotide #: NM_001191016.2
• UniProt Accession #: Q6UXS9; D6RBN7
• Molecular Weight: 29,399 Da
• NCBI Official Full Name: inactive caspase-12
• NCBI Official Synonym Full Names: caspase 12 (gene/pseudogene)
• NCBI Official Symbol: CASP12
• NCBI Official Synonym Symbols: CASP-12; CASP12P1
• NCBI Protein Information: inactive caspase-12
• UniProt Protein Name: Inactive caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: CASP12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_HUMAN

NCBI Description

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.