Kruppel Like Factor 4, Gut (KLF4) Recombinant Protein | KLF4 recombinant protein

Recombinant Kruppel Like Factor 4, Gut (KLF4)

Cat. Num. AAA2018350

• Gene Names: Klf4; EZF; Zie; Gklf
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Kruppel Like Factor 4; Gut (KLF4); Recombinant Kruppel Like Factor 4; KLF4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- S SPSVISVSKG SPDGSHPVVV APYSGGPPRM CPKIKQEAVP SCTVSRSLEA HLSAGPQLSN GHRPNTHDFP LGRQLPTRTT PTLSPEELLN SRDCHPGLPL PPGFHPHPGP NYPPFLPDQM QSQVPSLHYQ ELMPPGSCLP EEPKPKRGRR SWPRKRTATH TCDYAGCGKT YTKSSHLKAH LRTHTGEKPYHCDWDGCGWK FARSDELTRH YRKHTGHRPF QCQKCDRAFS RSDHLALH
• Sequence Length: 483

• Applicable Applications for KLF4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser240~His478 (Accession # Q60793) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.5
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 171543888
• NCBI GeneID: 16600
• NCBI Accession #: NP_034767.2
• NCBI GenBank Nucleotide #: NM_010637.3
• UniProt Accession #: Q60793; P70421; Q3U2D6; Q3URS6; Q78K30; Q9R255
• Molecular Weight: 30.4kDa
• NCBI Official Full Name: Krueppel-like factor 4
• NCBI Official Synonym Full Names: Kruppel-like factor 4 (gut)
• NCBI Official Symbol: Klf4
• NCBI Official Synonym Symbols: EZF; Zie; Gklf
• NCBI Protein Information: Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF)
• UniProt Protein Name: Krueppel-like factor 4
• Protein Family: Krueppel-like factor
• UniProt Gene Name: Klf4
• UniProt Synonym Gene Names: Ezf; Gklf; Zie
• UniProt Entry Name: KLF4_MOUSE

Uniprot Description

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; chromatin; nucleus

Molecular Function: nucleic acid binding; DNA binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; double-stranded DNA binding; beta-catenin binding; phosphoinositide 3-kinase regulator activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; negative regulation of caspase activity; positive regulation of protein metabolic process; regulation of phosphoinositide 3-kinase activity; positive regulation of hemoglobin biosynthetic process; response to organic substance; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; post-embryonic hemopoiesis; regulation of cell differentiation; epidermis morphogenesis; cell differentiation; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; transcription, DNA-dependent; stem cell maintenance; positive regulation of telomerase activity; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; inhibition of NF-kappaB transcription factor; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of phosphoinositide 3-kinase cascade