Rabbit CARP Polyclonal Antibody | anti-CARP antibody

CARP (A243) Polyclonal Antibody

Cat. Num. AAA3002138

• Gene Names: ANKRD1; ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CARP; Polyclonal Antibody; CARP (A243) Polyclonal Antibody; Ankyrin repeat domain-containing protein 1; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; ANKRD1; C193; HA1A2; anti-CARP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CARP (A243) polyclonal antibody detects endogenous levels of CARP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 319

• Applicable Applications for anti-CARP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 213-273 of Human CARP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CARP (A243) polyclonal antibody at 1:500 dilutionLane1:The Muscle tissue lysate of Rat (30ug) Lane2:The Muscle tissue lysate of Mouse (40ug))

Related Product Information for anti-CARP antibody

CARphospho-1 (cell division cycle and apoptosis regulator 1), also known as CCAR1 or DIS, is a 1, 150 amino acid protein that localizes to the perinuclear region of the cytoplasm and contains one SAP domain. Expressed in several epithelial cancer cell lines, including breast, colon, prostate and leukemia, CARphospho-1 is involved in apoptotic signaling, as well as in cell cycle progression and cell proliferation via interaction with c-Myc and cyclin B1. CARphospho-1 is subject to DNA damage-induced phosphorylation, probably by ATM or ATR. The gene encoding CARphospho-1 maps to human chromosome 10, which houses over 1, 200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-MarieTooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolmans syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria

NCBI and Uniprot Product Information

• NCBI GI #: 38327522
• NCBI GeneID: 27063
• NCBI Accession #: NP_055206.2
• NCBI GenBank Nucleotide #: NP_055206.2
• UniProt Accession #: Q15327
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: ankyrin repeat domain-containing protein 1
• NCBI Official Synonym Full Names: ankyrin repeat domain 1
• NCBI Official Symbol: ANKRD1
• NCBI Official Synonym Symbols: ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
• NCBI Protein Information: ankyrin repeat domain-containing protein 1
• UniProt Protein Name: Ankyrin repeat domain-containing protein 1
• UniProt Gene Name: ANKRD1
• UniProt Synonym Gene Names: C193; CARP; HA1A2
• UniProt Entry Name: ANKR1_HUMAN

NCBI Description

The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system. [provided by RefSeq, Jul 2008]

Uniprot Description

ANKRD1: May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells. Defects in ANKRD1 may be a cause of total anomalous pulmonary venous return (TAPVR). TAPVR is a rare congenital heart disease (CHD) in which the pulmonary veins fail to connect to the left atrium during cardiac development, draining instead into either the right atrium or one of its venous tributaries. This disease accounts for 1.5% of all CHDs and has a prevalence of approximately 1 out of 15'000 live births.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: nucleoplasm; I band; transcription factor complex; sarcomere; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; DNA binding; p53 binding; histone deacetylase binding; titin binding; transcription corepressor activity

Biological Process: positive regulation of protein secretion; positive regulation of apoptosis; cardiac muscle morphogensis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; sarcomere organization