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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using DNTT antibody.)

anti-Human DNTT Antibody | anti-DNTT antibody

DNTT Antibody

Gene Names
DNTT; TDT
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DNTT; DNTT Antibody; deoxynucleotidyltransferase; terminal; DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TDT; terminal addition enzyme; terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; terminal transferase; anti-DNTT antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
DNTT antibody detects endogenous levels of total DNTT protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
508
Applicable Applications for anti-DNTT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human DNTT.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using DNTT antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using DNTT antibody.)
References
Peterson R.C., J. Biol. Chem. 260:10495-10502(1985). Riley L.K., Proc. Natl. Acad. Sci. U.S.A. 85:2489-2493(1988). Ibe S., Genes Cells 6:815-824(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,408 Da
NCBI Official Full Name
DNA nucleotidylexotransferase isoform 2
NCBI Official Synonym Full Names
DNA nucleotidylexotransferase
NCBI Official Symbol
DNTT
NCBI Official Synonym Symbols
TDT
NCBI Protein Information
DNA nucleotidylexotransferase
UniProt Protein Name
DNA nucleotidylexotransferase
UniProt Gene Name
DNTT
UniProt Synonym Gene Names
; Terminal transferase

NCBI Description

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Research Articles on DNTT

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Product Notes

The DNTT dntt (Catalog #AAA852551) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The DNTT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNTT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:40000. Researchers should empirically determine the suitability of the DNTT dntt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNTT, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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