Carbonic Anhydrase II (CA2) Recombinant Protein | CA2 recombinant protein

Recombinant Carbonic Anhydrase II (CA2)

Cat. Num. AAA2011499

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Carbonic Anhydrase II (CA2); Recombinant Carbonic Anhydrase II (CA2); CA2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- MSHHWGYDSH NGPAHWHEHF PIANGERQSP IAISTKAARY DPALKPLSFS YDAGTAKAIV NNGHSFNVEF DDSSDKSVLQ GGALDGVYRL VQFHIHWGSC EGQGSEHTVD GVKYDAELHI VHWNVKYGKF AEALKHPDGL AVVGIFMKVG NAKPEIQKVV DALNSIQTKG KQASFTNFDP TGLLPPCRDY WTYPGSLTTP PLHECVIWHV LKEPITVSSE QMCKLRGLCF SAENEPVCRM VDNWRPCQPL KSREVRASFQ
• Sequence Length: 260

• Applicable Applications for CA2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Chicken (Gallus)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln260 (Accession # P07630) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46048696
• NCBI GeneID: 396257
• NCBI Accession #: NP_990648.1
• NCBI GenBank Nucleotide #: NM_205317.1
• UniProt Accession #: P07630
• Molecular Weight: 30.5kDa
• NCBI Official Full Name: carbonic anhydrase 2
• NCBI Official Synonym Full Names: carbonic anhydrase II
• NCBI Official Symbol: CA2
• NCBI Protein Information: carbonic anhydrase 2; CA-II; carbonate dehydratase II
• UniProt Protein Name: Carbonic anhydrase 2
• Protein Family: Beta-carotene hydroxylase
• UniProt Gene Name: CA2
• UniProt Synonym Gene Names: CA-II
• UniProt Entry Name: CAH2_CHICK

Uniprot Description

Function: Essential for bone resorption and osteoclast differentiation

By similarity. Reversible hydration of carbon dioxide.

Catalytic activity: H2CO3 = CO2 + H2O.

Cofactor: Zinc.

Enzyme regulation: Inhibited by acetazolamide

By similarity.

Subcellular location: Cytoplasm. Cell membrane

By similarity.

Sequence similarities: Belongs to the alpha-carbonic anhydrase family.