Rabbit anti-Human CARMA2 Polyclonal Antibody | anti-CARD14 antibody

CARMA2 Antibody

Cat. Num. AAA150505

• Gene Names: CARD14; PRP; PSS1; BIMP2; CARMA2; PSORS2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: CARMA2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CARMA2; Polyclonal Antibody; CARMA2 Antibody; PRP; PSS1; BIMP2; PSORS2; Caspase recruitment domain-containing protein 14; CARD-containing MAGUK protein 2; Carma 2; caspase recruitment domain family; member 14; anti-CARD14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CARMA2 antibody is human specific. At least three isoforms of CARMA2 are known to exist; this antibody will only detect isoform 1. CARMA2 antibody is predicted not to cross-react with other CARMA proteins.
• Purity/Purification: CARMA2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1004

• Applicable Applications for anti-CARD14 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: CARMA2 antibody can be used for detection of CARMA2 by immunohistochemistry at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CARMA2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human CARMA2.
• Buffer: CARMA2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CARMA2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunohistochemistry (IHC)

(Immunohistochemistry of CARMA2 in human placenta tissue with CARMA2 antibody at 5 μg/mL.)

Related Product Information for anti-CARD14 antibody

CARMA2 Antibody: CARMA proteins belong to the membrane-associated guan-ylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA2 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-κB activation. When expressed in cells, this protein activated NF-κB and induced the phosphorylation of BCL10 Alternative splicing of CARMA2 results in isoforms that possess differential effects on NF-κB activation and endoplasmic reticulum stress-induced cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 332801087
• NCBI GeneID: 79092
• NCBI Accession #: NP_077015
• NCBI GenBank Nucleotide #: NM_024110.4
• UniProt Accession #: Q9BXL6; Q9BVB5; B8QQJ3
• Molecular Weight: 48,505 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 14 isoform 1
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 14
• NCBI Official Symbol: CARD14
• NCBI Official Synonym Symbols: PRP; PSS1; BIMP2; CARMA2; PSORS2
• NCBI Protein Information: caspase recruitment domain-containing protein 14; CARD-containing MAGUK protein 2; bcl10-interacting maguk protein 2
• UniProt Protein Name: Caspase recruitment domain-containing protein 14
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD14
• UniProt Synonym Gene Names: CARMA2; Carma 2
• UniProt Entry Name: CAR14_HUMAN

NCBI Description

This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

CARD14: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; plasma membrane

Molecular Function: CARD domain binding

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of apoptosis

Disease: Psoriasis 2; Pityriasis Rubra Pilaris