Rabbit anti-Human RIP Polyclonal Antibody | anti-RIP antibody

RIP Rabbit pAb

Cat. Num. AAA4756958

• Gene Names: RIPK1; RIP; RIP1
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: RIP; Polyclonal Antibody; RIP Rabbit pAb; Receptor-interacting serine/threonine-protein kinase 1; receptor interacting serine/threonine kinase 1; RIP1; IMD57; RIP-1; anti-RIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-RIP antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:2000; FC/FACS: 1:20
• Immunogen: Recombinant protein of human RIP
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Cell membrane, Cytoplasm, Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of RIP in K562 lysates using RIP antibody.Predicted band size:76kDa.Observed band size:76kDa.)

Related Product Information for anti-RIP antibody

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage (PubMed:11101870, PubMed:17389591, PubMed:19524512, PubMed:19524513). Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor (PubMed:11101870, PubMed:17389591, PubMed:19524512, PubMed:19524513). Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (PubMed:17389591). Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules (PubMed:15258597). Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death (PubMed:15258597). RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (PubMed:19524513, PubMed:19524512).
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]

Product Categories/Family for anti-RIP antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 57242761
• NCBI GeneID: 8737
• NCBI Accession #: NP_003795
• NCBI GenBank Nucleotide #: NM_003804.3
• UniProt Accession #: Q13546; Q13180; Q59H33; A0AV89; B2RAG1; B4E3F9
• Molecular Weight: 70,733 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 1
• NCBI Official Synonym Full Names: receptor (TNFRSF)-interacting serine-threonine kinase 1
• NCBI Official Symbol: RIPK1
• NCBI Official Synonym Symbols: RIP; RIP1
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 1; RIP-1; cell death protein RIP; receptor interacting protein; receptor-interacting protein 1; serine/threonine-protein kinase RIP
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 1
• Protein Family: Riparin
• UniProt Gene Name: RIPK1
• UniProt Synonym Gene Names: RIP; RIP1; RIP-1
• UniProt Entry Name: RIPK1_HUMAN

Uniprot Description

RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: mitochondrion; cytoplasm; cytosol; receptor complex; lipid raft

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; protein complex binding; ATP binding; protein kinase activity

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; positive regulation of apoptosis; apoptosis; MyD88-independent toll-like receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; tumor necrosis factor-mediated signaling pathway; cellular protein catabolic process; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; protein homooligomerization