Rabbit Carbonic Anhydrase III Polyclonal Antibody | anti-CA3 antibody

Anti-Carbonic Anhydrase III Antibody, Rabbit Polyclonal

Cat. Num. AAA8103750

• Gene Names: Car3; Ca3; Car-3; BB219044
• Reactivity: Mouse; Predicted to React with: Human, Rat, Bovine, Pig (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Carbonic Anhydrase III; Polyclonal Antibody; Anti-Carbonic Anhydrase III Antibody; Rabbit Polyclonal; Carbonic Anhydrase III/CA3 Antibody; Rabbit PAb; Antigen Affinity Purified; carbonic anhydrase III; anti-CA3 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human, Rat, Bovine, Pig (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Carbonic Anhydrase III
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CA3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Carbonic Anhydrase III/CA3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Carbonic Anhydrase III/CA3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CA3 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lane B: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:30 kDa)

Immunoprecipitation (IP)

(CA3 was immunoprecipitated using: Lane A:0.5 mg Mouse pancreas Whole Cell Lysate 4 uL anti-CA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CA3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size :28 kDa)

Related Product Information for anti-CA3 antibody

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called beta-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to thealpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.

References

Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596-601. Sowden J. et al., 1998, Gene 214:157-65. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899-903. Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62. Bataller, L. et al., 2004, Ann Neurol 56 (4): 575-9.

NCBI and Uniprot Product Information

• NCBI GI #: 31982861
• NCBI GeneID: 12350
• NCBI Accession #: NP_031632.2
• NCBI GenBank Nucleotide #: NM_007606.3
• UniProt Accession #: P16015; Q9ERN8
• Molecular Weight: 29,366 Da
• NCBI Official Full Name: carbonic anhydrase 3
• NCBI Official Synonym Full Names: carbonic anhydrase 3
• NCBI Official Symbol: Car3
• NCBI Official Synonym Symbols: Ca3; Car-3; BB219044
• NCBI Protein Information: carbonic anhydrase 3
• UniProt Protein Name: Carbonic anhydrase 3
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: Ca3
• UniProt Synonym Gene Names: Car3; CA-III

Uniprot Description

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 3 A1|3 3.22 cM

Cellular Component: cytoplasm; cytosol

Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding; nickel ion binding; phosphoric monoester hydrolase activity; zinc ion binding

Biological Process: one-carbon metabolic process; response to oxidative stress