Carbonic Anhydrase III / CA3 Recombinant Protein | CA3 recombinant protein

Recombinant Human Carbonic Anhydrase III / CA3 Protein (His tag)

Cat. Num. AAA2546219

• Gene Names: CA3; Car3; CAIII
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase III / CA3; Recombinant Human Carbonic Anhydrase III / CA3 Protein (His tag); CAIII; Car3; CA3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
• Sequence Length: 260

• Application Notes: The recombinant human CA3 consisting of 266 amino acids and has a calculated molecular mass of 30.4 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CA3 recombinant protein

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called beta-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.

Description: A DNA sequence encoding the human CA3 (NP_005172.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4885099
• NCBI GeneID: 761
• NCBI Accession #: NP_005172.1
• NCBI GenBank Nucleotide #: NM_005181.3
• UniProt Accession #: P07451; O60842; B2R867; B3KUC8
• Molecular Weight: 29,557 Da
• NCBI Official Full Name: carbonic anhydrase 3
• NCBI Official Synonym Full Names: carbonic anhydrase 3
• NCBI Official Symbol: CA3
• NCBI Official Synonym Symbols: Car3; CAIII
• NCBI Protein Information: carbonic anhydrase 3
• UniProt Protein Name: Carbonic anhydrase 3
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA3
• UniProt Synonym Gene Names: CA-III

NCBI Description

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Uniprot Description

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity

Biological Process: bicarbonate transport