Western Blot (WB)
(Western Blot: Lane1: Mouse Skeletal Muscle Tissue; Lane2: Rat Skeletal Muscle Tissue.)
Rabbit anti-Human Carbonic Anhydrase III, Muscle Specific (CA3) Polyclonal Antibody | anti-CA3 antibody
Polyclonal Antibody to Carbonic Anhydrase III, Muscle Specific (CA3)
Gene Names
Car3; Ca3; Car-3; BB219044
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific Affinity Chromatography
Synonyms
Carbonic Anhydrase III; Muscle Specific (CA3); Polyclonal Antibody; Polyclonal Antibody to Carbonic Anhydrase III; anti-CA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a Rabbit polyclonal antibody raised against CA3. It has been selected for its ability to recognize CA3 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-CA3 antibody
WB; IHC; ICC; IP
Application Notes
Western blotting: 0.5-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant CA3 (Ala2~Lys260) expressed in E.coli
Cross Reactivity
Human, Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2069771
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Lane1: Mouse Skeletal Muscle Tissue; Lane2: Rat Skeletal Muscle Tissue.)
Western Blot (WB)
(Western Blot: Lane1: Mouse Skeletal Muscle Tissue; Lane2: Rat Skeletal Muscle Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CA3, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CA3, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,366 Da
NCBI Official Full Name
carbonic anhydrase 3
NCBI Official Synonym Full Names
carbonic anhydrase 3
NCBI Official Symbol
Car3
NCBI Official Synonym Symbols
Ca3; Car-3; BB219044
NCBI Protein Information
carbonic anhydrase 3
UniProt Protein Name
Carbonic anhydrase 3
Protein Family
UniProt Gene Name
Ca3
UniProt Synonym Gene Names
Car3; CA-III
Uniprot Description
CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase
Chromosomal Location of Human Ortholog: 3 A1|3 3.22 cM
Cellular Component: cytoplasm; cytosol
Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding; nickel ion binding; phosphoric monoester hydrolase activity; zinc ion binding
Biological Process: one-carbon metabolic process; response to oxidative stress
Research Articles on CA3
Similar Products
Product Notes
The CA3 ca3 (Catalog #AAA2001566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Carbonic Anhydrase III, Muscle Specific (CA3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase III, Muscle Specific (CA3) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-5µg/mL Immunocytochemistry in formalin fixed cells: 5-20µg/mL Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL Immunohistochemistry in paraffin section: 5-20µg/mL Optimal working dilutions must be determined by end user . Researchers should empirically determine the suitability of the CA3 ca3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase III, Muscle Specific (CA3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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