Goat CAPG / MCP Polyclonal Antibody | anti-CAPG antibody

Goat Polyclonal to Human CAPG / MCP

Cat. Num. AAA248076

• Gene Names: CAPG; MCP; AFCP; HEL-S-66
• Reactivity: Guinea pig, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CAPG / MCP; Polyclonal Antibody; Goat Polyclonal to Human CAPG / MCP; Anti-CAPG / MCP Antibody (aa205-217) IHC-plus; CAPG; AFCP; Actin-regulatory protein CAP-G; Actin regulatory protein CAP-G; Gelsolin-like capping protein; MCP; Macrophage capping protein; Macrophage-capping protein; Human CAPG; anti-CAPG antibody

• Host: Goat
• Reactivity: Guinea pig, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Human CAPG / MCP. This antibody is expected to recognize isoform 1 (NP_001738.2) only. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 348

• Applicable Applications for anti-CAPG antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 38kD band observed in lysates of cell line U937 and of Human Spleen (calculated MW of 38.5kD according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band...
• Target Species: Human
• Immunogen Species: CAPG antibody was raised against Human
• Immunogen: CAPG antibody was raised against peptide with sequence C-KAQVEIVTDGEEP, from the internal region of the protein sequence according to NP_001738.2.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CAPG / MCP antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CAPG / MCP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(CAPG Antibody (0.3µg/ml) staining of Periheral Blood Lymphocytes, (lane B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(CAPG Antibody (0.1µg/ml) staining of Mouse (A) and Rat Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(CAPG Antibody (0.3µg/ml) staining of U937 (A) and Human Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-CAPG antibody

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 38kD band observed in lysates of cell line U937 and of Human Spleen (calculated MW of 38.5kD according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kD. Recommended concentration: 0.1-0.3 ug/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 63252913
• NCBI GeneID: 822
• NCBI Accession #: NP_001738.2
• NCBI GenBank Nucleotide #: NM_001747.3
• UniProt Accession #: P40121; Q53SA7; B8ZZS7; D6W5K8
• NCBI Official Full Name: macrophage-capping protein isoform 1
• NCBI Official Synonym Full Names: capping protein (actin filament), gelsolin-like
• NCBI Official Symbol: CAPG
• NCBI Official Synonym Symbols: MCP; AFCP; HEL-S-66
• NCBI Protein Information: macrophage-capping protein; macrophage capping protein; gelsolin-like capping protein; actin regulatory protein CAP-G; actin-regulatory protein CAP-G; epididymis secretory protein Li 66
• UniProt Protein Name: Macrophage-capping protein
• Protein Family: Protein
• UniProt Gene Name: CAPG
• UniProt Synonym Gene Names: AFCP; MCP
• UniProt Entry Name: CAPG_HUMAN

NCBI Description

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

CAPG: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. Belongs to the villin/gelsolin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: F-actin capping protein complex; melanosome; nucleus

Molecular Function: actin binding

Biological Process: protein complex assembly; cell projection biogenesis; barbed-end actin filament capping