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Immunohistochemistry (IHC) (Anti-CAPG/MCP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Goat CAPG Polyclonal Antibody | anti-CAPG antibody

Anti-CAPG Antibody (aa147-159)

Gene Names
CAPG; MCP; AFCP; HEL-S-66
Reactivity
Human
Predicted: Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
CAPG; Polyclonal Antibody; Anti-CAPG Antibody (aa147-159); Mouse Monoclonal [clone 1F1] (IgG1; k) to Human CAPG; AFCP; Actin-regulatory protein CAP-G; Actin regulatory protein CAP-G; Gelsolin-like capping protein; MCP; Macrophage capping protein; Macrophage-capping protein; anti-CAPG antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human
Predicted: Monkey
Clonality
Polyclonal
Specificity
Human CAPG/MCP. This antibody is expected to recognize both reported isoforms (NP_001738.2; NP_001243069.1). Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
348
Applicable Applications for anti-CAPG antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ELISA: 1:64000
IHC-P: 5 ug/ml
WB: 0.3-1 ug/ml
Immunogen
Synthetic peptide C-NIRATERALNWDS from an internal region of human CAPG/MCP (NP_001738.2; NP_001243069.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Ferret, Elephant, Panda, Rabbit, Pig, Guinea pig (92%); Sheep, Bovine, Horse, Platypus (85%).
Conjugation
Unconjugated
Epitope
aa147-159
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CAPG/MCP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-CAPG/MCP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (WB)

(Anti-CAPG/MCP antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (WB) (Anti-CAPG/MCP antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(CAPG antibody (0.3 ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) (CAPG antibody (0.3 ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-CAPG antibody
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May playan important role in macrophage function. May play a role in regulating cytoplasmic and /or nuclear structures through potential interactions with actin. May bind DNA.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
822
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,857 Da
NCBI Official Full Name
macrophage-capping protein isoform 1
NCBI Official Synonym Full Names
capping actin protein, gelsolin like
NCBI Official Symbol
CAPG
NCBI Official Synonym Symbols
MCP; AFCP; HEL-S-66
NCBI Protein Information
macrophage-capping protein
UniProt Protein Name
Macrophage-capping protein
Protein Family
UniProt Gene Name
CAPG
UniProt Synonym Gene Names
AFCP; MCP

NCBI Description

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.CautionThis protein was originally thought to be a DNA-binding protein with a helix-loop-helix domain.

Research Articles on CAPG

Similar Products

Product Notes

The CAPG capg (Catalog #AAA2401058) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CAPG Antibody (aa147-159) reacts with Human Predicted: Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CAPG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:64000 IHC-P: 5 ug/ml WB: 0.3-1 ug/ml. Researchers should empirically determine the suitability of the CAPG capg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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