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Western Blot (WB) (Western Blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution.)

Rabbit CAMKIV Polyclonal Antibody | anti-CAMKIV antibody

CAMKIV Polyclonal Antibody

Gene Names
CAMK4; caMK; CaMKIV; CaMK IV; CaMK-GR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
CAMKIV; Polyclonal Antibody; CAMKIV Polyclonal Antibody; CaMK IV; CAMK4; CAMK; CAMK-GR; Brain Ca(2+) calmodulin dependent protein kinase type 4; Brain Ca(2+) calmodulin dependent protein kinase type IV; Brain Ca++-calmodulin dependent protein kinase type IV; Calcium / calmodulin dependent protein kinase type 4 catalytic chain; Calcium / calmodulin dependent protein kinase type IV catalytic chain; Calcium/calmodulin dependent protein kinase IV; Calcium/calmodulin dependent protein kinase type IV; Calcium/calmodulin-dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CAM kinase-GR; CaMK 4; CAMK GR; Camk4; CaMKGR; IV; KCC4; MGC36771; anti-CAMKIV antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CAMKIV polyclonal antibody detects endogenous levels of CAMKIV protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
473
Applicable Applications for anti-CAMKIV antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein corresponding to the C-terminus of human CAMKIV.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution.)

Western Blot (WB) (Western Blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-CAMKIV antibody
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated alpha, beta, gamma and delta, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
814
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52kDa
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type IV isoform 1
NCBI Official Synonym Full Names
calcium/calmodulin dependent protein kinase IV
NCBI Official Symbol
CAMK4
NCBI Official Synonym Symbols
caMK; CaMKIV; CaMK IV; CaMK-GR
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type IV
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type IV
UniProt Gene Name
CAMK4
UniProt Synonym Gene Names
CAMK; CAMK-GR; CAMKIV; CaMK IV
UniProt Entry Name
KCC4_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; regulation of T cell differentiation in the thymus; nucleocytoplasmic transport; myeloid dendritic cell differentiation; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production

Research Articles on CAMKIV

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Product Notes

The CAMKIV camk4 (Catalog #AAA3012352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAMKIV Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CAMKIV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CAMKIV camk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMKIV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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