Rabbit anti-Human JIK Polyclonal Antibody | anti-JIK antibody

JIK, CT (JNK/SAPK-inhibitory Kinase, Jun Kinase Inhibitory Kinase, Cutaneous T-cell Lymphoma-associated Antigen HD-CL-09, CTCL-associated Antigen HD-CL-09, Dendritic Cell-derived Protein Kinase, DPK, KDS, Kinase from Chicken Homolog A, hKFC-A, MAP3K18, Se

Cat. Num. AAA6483735

• Gene Names: TAOK3; DPK; JIK; MAP3K18
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JIK; Polyclonal Antibody; CT (JNK/SAPK-inhibitory Kinase; Jun Kinase Inhibitory Kinase; Cutaneous T-cell Lymphoma-associated Antigen HD-CL-09; CTCL-associated Antigen HD-CL-09; Dendritic Cell-derived Protein Kinase; DPK; KDS; Kinase from Chicken Homolog A; hKFC-A; MAP3K18; Se; DKFZp666H245; EC=2.7.11.1; FLJ31808; anti-JIK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAOK3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-JIK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human TAOK3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TAOK3 (arrow) using rabbit polyclonal TAOK3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAOK3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TAOK3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-JIK antibody

Antibodies; Protein Kinases

References

1. Hartmann, T.B., Br. J. Dermatol. 150(2), 252-258 (2004). 2. Yustein, J.T., Oncogene 22(40), 6129-6141 (2003). 3. Yoneda, T., J. Biol. Chem. 276(17), 13935-13940 (2001). 4. Zhang, W., Biochem. Biophys. Res. Commun. 274(3), 872-879 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 148746218
• NCBI GeneID: 51347
• NCBI Accession #: NP_057365
• NCBI GenBank Nucleotide #: NM_016281
• UniProt Accession #: Q9H2K8; Q658N1; Q8IUM4; Q9HC79; Q9NZM9; Q9UHG7
• Molecular Weight: 105,406 Da
• NCBI Official Full Name: serine/threonine-protein kinase TAO3
• NCBI Official Synonym Full Names: TAO kinase 3
• NCBI Official Symbol: TAOK3
• NCBI Official Synonym Symbols: DPK; JIK; MAP3K18
• NCBI Protein Information: serine/threonine-protein kinase TAO3; CTCL-associated antigen HD-CL-09; JNK/SAPK-inhibitory kinase; STE20-like kinase; cutaneous T-cell lymphoma-associated antigen HD-CL-09; dendritic cell-derived protein kinase; hKFC-A; jun kinase-inhibitory kinase; kina
• UniProt Protein Name: Serine/threonine-protein kinase TAO3
• UniProt Gene Name: TAOK3
• UniProt Synonym Gene Names: DPK; JIK; KDS; MAP3K18; CTCL-associated antigen HD-CL-09; hKFC-A
• UniProt Entry Name: TAOK3_HUMAN

Uniprot Description

TAO3: Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress- activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF). Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; TAO subfamily

Chromosomal Location of Human Ortholog: 12q

Cellular Component: cytoplasm; plasma membrane

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein kinase inhibitor activity; MAP kinase kinase kinase activity; ATP binding

Biological Process: negative regulation of JNK cascade; positive regulation of JNK activity; activation of MAPKK activity; protein amino acid autophosphorylation; mitotic cell cycle G2/M transition DNA damage checkpoint; MAPKKK cascade; negative regulation of protein kinase activity; positive regulation of JNK cascade; DNA repair; response to DNA damage stimulus; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade