Rabbit anti-Human, Rat UBE2W Polyclonal Antibody | anti-UBE2W antibody

UBE2W Antibody

Cat. Num. AAA9417020

• Gene Names: UBE2W; UBC16; UBC-16
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: UBE2W; Polyclonal Antibody; UBE2W Antibody; UBC16; UBC-16; anti-UBE2W antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total UBE2W protein.
• Purity/Purification: Antigen affinity purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 1.4mg/ml (varies by lot)
• Sequence Length: 191

• Applicable Applications for anti-UBE2W antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot: 1:500-2000; Immunohistochemistry: 1: 100-250
• Immunogen: Full length fusion protein
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

SDS-Page

(Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-2: 231 cell and Human cerebrum tissue lysates, Primary antibody:UBE2W antibody at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 4 minutes)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using UBE2W Antibody at dilution 1/100, on the right is treated with fusion protein. (Original magnification: x200))

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using UBE2W Antibody at dilution 1/100, on the right is treated with fusion protein. (Original magnification: x200))

Related Product Information for anti-UBE2W antibody

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-UBE2W antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 402169567
• NCBI GeneID: 55284
• NCBI Accession #: NP_001001481.2
• NCBI GenBank Nucleotide #: NM_001001481.2
• UniProt Accession #: Q96B02; Q1XBE0; Q9H823; Q9HAG6; Q9NV07; B4DIV1
• Molecular Weight: 20,689 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 W isoform 1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 W
• NCBI Official Symbol: UBE2W
• NCBI Official Synonym Symbols: UBC16; UBC-16
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 W
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 W
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2W
• UniProt Synonym Gene Names: UBC16; UBC-16
• UniProt Entry Name: UBE2W_HUMAN

NCBI Description

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: misfolded or incompletely synthesized protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination