Goat CAMK2A / CaMKII Alpha Polyclonal Antibody | anti-CAMK2A antibody

Goat Polyclonal to Human CAMK2A / CaMKII Alpha

Cat. Num. AAA247418

• Gene Names: CAMK2A; CAMKA
• Reactivity: Bat, Bovine, Dog, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using immunizing peptide.
• Synonyms: CAMK2A / CaMKII Alpha; Polyclonal Antibody; Goat Polyclonal to Human CAMK2A / CaMKII Alpha; Anti-CAMK2A / CaMKII Alpha Antibody (Internal) IHC-plus; CAMK2A; AlphaCaMKII; CaM-kinase II alpha chain; CaMK-II subunit alpha; CAMKA; CaM kinase II alpha; CaMK II alpha; CaMK-II alpha subunit; CaMKIINalpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMKII alpha; CaMKIIalpha; KIAA0968; Human CAMK2A; CaMKII Alpha; anti-CAMK2A antibody

• Host: Goat
• Reactivity: Bat, Bovine, Dog, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Human CAMK2A. This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.5% BSA, 0.02% Sodium Azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 489

• Applicable Applications for anti-CAMK2A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Approx 55+65kDa bands observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2 and of 54.1 according to Human NP_741960.1 and Mouse NP_803126.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/ml.
• Target: CAMK2A/CaMKII Alpha
• Epitope: aa455-468
• Immunogen Species: Raised against human
• Immunogen: CAMK2A / CaMKII Alpha antibody was raised against peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_741960.1
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store -20°C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-CAMK2A antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CAMK2A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody (0.1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-CAMK2A antibody

CAMK2Alpha, together with the beta, delta, and gamma subunits, constititute the CAMK2 heteromultimeric holoenzyme serine/threonine kinase. CAMK2A has been implicated in learning, where it potentiates synaptic transmission by phosphorylating AMPA receptors. Variants are produced by alternative splicing.

Product Categories/Family for anti-CAMK2A antibody

Family: Protein Kinase; Subfamily: CAMK2

NCBI and Uniprot Product Information

• NCBI GI #: 25952114
• NCBI GeneID: 815
• NCBI Accession #: NP_057065.2
• NCBI GenBank Nucleotide #: NM_015981.3
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: presynaptic membrane; nucleoplasm; mitochondrion; plasma membrane; cell junction; nucleus; cytosol

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein binding; glutamate receptor binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; cytokine and chemokine mediated signaling pathway; calcium ion transport; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor