Western Blot (WB)
((0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat CAMK2A Polyclonal Antibody | anti-CAMK2A antibody
Goat anti-CAMK2A Antibody
Immunohistochemistry: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 65kDa bands observed in Human Brain (Cerebellum) and Mouse and Rat Brain lysates and an additional 50kDa in the rodent Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2 and of 54.1 according to Human NP_741960.1 to Mouse NP_803126.1 and to Rat NP_037052.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
Uniprot Description
CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Protein kinase, CAMK; CAMK group; CAMK2 family
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: cell junction; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane; presynaptic membrane
Molecular Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; glutamate receptor binding; kinase activity; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; Ras guanyl-nucleotide exchange factor activity
Biological Process: activation of NF-kappaB transcription factor; calcium ion transport; G1/S transition of mitotic cell cycle; MAPKKK cascade; peptidyl-serine phosphorylation; positive regulation of calcium ion transport; positive regulation of GTPase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; regulation of neurotransmitter secretion; Wnt receptor signaling pathway, calcium modulating pathway
Research Articles on CAMK2A
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Product Notes
The CAMK2A camk2a (Catalog #AAA421825) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CAMK2A Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CAMK2A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration, 3-6ug/ml. Western Blot: Approx 65kDa bands observed in Human Brain (Cerebellum) and Mouse and Rat Brain lysates and an additional 50kDa in the rodent Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2 and of 54.1 according to Human NP_741960.1 to Mouse NP_803126.1 and to Rat NP_037052.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CAMK2A camk2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PRTAQSEETR VWHR. It is sometimes possible for the material contained within the vial of "CAMK2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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