Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 2ug/mL Rabbit Anti-Rat CGRP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Calcitonin Gene Related Peptide (CGRP) Polyclonal Antibody | anti-CGRP antibody
Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Calcitonin Gene Related Peptide (CGRP); Polyclonal Antibody; Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP); anti-CGRP antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CGRP. It has been selected for its ability to recognize CGRP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- MDFWKFFPFL ALSSMWVLCL ASSLQAAPFR SALESSLDLG TLSDQEKHLL LAALIQDYEQ KARKLEQEEQ ETEGSRKGSS SSVISQKRSC NTATCVTHRL AGLLRRSGGV VKDNFVPTNV GSKAFGRRRR DLRV
MGHHHHHHSGSEF- MDFWKFFPFL ALSSMWVLCL ASSLQAAPFR SALESSLDLG TLSDQEKHLL LAALIQDYEQ KARKLEQEEQ ETEGSRKGSS SSVISQKRSC NTATCVTHRL AGLLRRSGGV VKDNFVPTNV GSKAFGRRRR DLRV
Applicable Applications for anti-CGRP antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CGRP (Met1~Val134) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049023
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 2ug/mL Rabbit Anti-Rat CGRP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 2ug/mL Rabbit Anti-Rat CGRP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,965 Da
NCBI Official Full Name
calcitonin gene-related peptide 2
NCBI Official Synonym Full Names
calcitonin-related polypeptide, beta
NCBI Official Symbol
Calcb
NCBI Protein Information
calcitonin gene-related peptide 2
UniProt Protein Name
Calcitonin gene-related peptide 2
UniProt Gene Name
Calcb
UniProt Synonym Gene Names
CGRP-II
NCBI Description
may play a role in regulating transduction of sensory and motor information [RGD, Feb 2006]
Uniprot Description
CALCB: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. Belongs to the calcitonin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q35
Cellular Component: extracellular region
Molecular Function: hormone activity
Research Articles on CGRP
Similar Products
Product Notes
The CGRP calcb (Catalog #AAA2003224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calcitonin Gene Related Peptide (CGRP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CGRP calcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- MDFWKFFPFL ALSSMWVLCL ASSLQAAPFR SALESSLDLG TLSDQEKHLL LAALIQDYEQ KARKLEQEEQ ETEGSRKGSS SSVISQKRSC NTATCVTHRL AGLLRRSGGV VKDNFVPTNV GSKAFGRRRR DLRV. It is sometimes possible for the material contained within the vial of "Calcitonin Gene Related Peptide (CGRP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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