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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human LH Standard Curve.)

Human LH ELISA Kit | LH elisa kit

Human LH ELISA Kit

Gene Names
LHB; CGB4; HH23; LSH-B; LSH-beta
Reactivity
Human
Synonyms
LH; Human LH ELISA Kit; LH-B; LHB; LSH-B; LSH-beta; LSHB_HUMAN; Luteinizing hormone subunit beta; Lutropin beta chain; Lutropin subunit beta; LH elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human LH ELISA Kit has high sensitivity and excellent specificity for detection of Human LH. No significant cross-reactivity or interference between Human LH and analogues was observed.
Sequence Length
5
Assay Type
Sandwich ELISA (Quantitative)
Samples
Serum, Plasma, Other Biological Fluids
Detection Range
2 IU/L-75 IU/L
Detection Method
Colorimetric
Limit of Detection
0.5 IU/L
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human LH Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human LH Standard Curve.)
Related Product Information for LH elisa kit
Description: Human LH ELISA Kit employs a two-site sandwich ELISA to quantitate LH in samples. An antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for LH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormonereleasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
luteinizing hormone beta subunit (LHbeta), partial
NCBI Official Synonym Full Names
luteinizing hormone beta polypeptide
NCBI Official Symbol
LHB
NCBI Official Synonym Symbols
CGB4; HH23; LSH-B; LSH-beta
NCBI Protein Information
lutropin subunit beta
UniProt Protein Name
Lutropin subunit beta
UniProt Gene Name
LHB
UniProt Synonym Gene Names
Lutropin beta chain; LH-B; LSH-B; LSH-beta
UniProt Entry Name
LSHB_HUMAN

NCBI Description

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. [provided by RefSeq, Jul 2008]

Uniprot Description

LHB: Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB). Hypogonadism is characterized by infertility and pseudohermaphroditism. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: progesterone biosynthetic process; cellular protein metabolic process; cell-cell signaling; male gonad development; peptide hormone processing; signal transduction

Disease: Fertile Eunuch Syndrome

Research Articles on LH

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Product Notes

The Human LH lhb (Catalog #AAA9711570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711570 ELISA Kit recognizes Human LH. It is sometimes possible for the material contained within the vial of "LH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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